Details
| Original language | English |
|---|---|
| Pages (from-to) | 448-453 |
| Number of pages | 6 |
| Journal | PLANT BREEDING |
| Volume | 129 |
| Issue number | 4 |
| Publication status | Published - Aug 2010 |
Abstract
A 600 bp fragment of the ethylene-receptor gene 2 (ETR2) was sequenced in Petunia and Calibrachoa genotypes and in putative intergeneric somatic hybrids. In total, 16 genus-specific single base pair polymorphisms (SNPs) were detected. These were used to construct, genus specific restriction maps and a Cleaved Amplified Polymorphic Sequence marker with specific 400 bp fragments for Petunia and 550 bp for Calibrachoa. This diagnostic and robust marker is suitable to distinguish Petunia and Calibrachoa and is a valuable tool for the identification of intergeneric somatic hybrids which are currently developed in model breeding programmes. Based on sequence data for the ETR2 fragment, phylogenetic analyses were conducted. Neighbour-Joining trees reveal distinct subgroups of ETR2 of each genus, therefore supporting recent results of molecular phylogenetic studies on Petunia and Calibrachoa. Sequences of putative hybrids are represented in both subgroups, confirming their hybrid status.
Keywords
- CAPS, Genus specific molecular marker, Hybrid selection, Protoplast fusion, SNP
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANT BREEDING, Vol. 129, No. 4, 08.2010, p. 448-453.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The ethylene 2 receptor gene as a robust molecular marker for intergeneric somatic hybrids between Petunia and Calibrachoa
AU - Rode, C.
AU - Winkelmann, T.
AU - Meyer, L.
AU - Debener, T.
PY - 2010/8
Y1 - 2010/8
N2 - A 600 bp fragment of the ethylene-receptor gene 2 (ETR2) was sequenced in Petunia and Calibrachoa genotypes and in putative intergeneric somatic hybrids. In total, 16 genus-specific single base pair polymorphisms (SNPs) were detected. These were used to construct, genus specific restriction maps and a Cleaved Amplified Polymorphic Sequence marker with specific 400 bp fragments for Petunia and 550 bp for Calibrachoa. This diagnostic and robust marker is suitable to distinguish Petunia and Calibrachoa and is a valuable tool for the identification of intergeneric somatic hybrids which are currently developed in model breeding programmes. Based on sequence data for the ETR2 fragment, phylogenetic analyses were conducted. Neighbour-Joining trees reveal distinct subgroups of ETR2 of each genus, therefore supporting recent results of molecular phylogenetic studies on Petunia and Calibrachoa. Sequences of putative hybrids are represented in both subgroups, confirming their hybrid status.
AB - A 600 bp fragment of the ethylene-receptor gene 2 (ETR2) was sequenced in Petunia and Calibrachoa genotypes and in putative intergeneric somatic hybrids. In total, 16 genus-specific single base pair polymorphisms (SNPs) were detected. These were used to construct, genus specific restriction maps and a Cleaved Amplified Polymorphic Sequence marker with specific 400 bp fragments for Petunia and 550 bp for Calibrachoa. This diagnostic and robust marker is suitable to distinguish Petunia and Calibrachoa and is a valuable tool for the identification of intergeneric somatic hybrids which are currently developed in model breeding programmes. Based on sequence data for the ETR2 fragment, phylogenetic analyses were conducted. Neighbour-Joining trees reveal distinct subgroups of ETR2 of each genus, therefore supporting recent results of molecular phylogenetic studies on Petunia and Calibrachoa. Sequences of putative hybrids are represented in both subgroups, confirming their hybrid status.
KW - CAPS
KW - Genus specific molecular marker
KW - Hybrid selection
KW - Protoplast fusion
KW - SNP
UR - http://www.scopus.com/inward/record.url?scp=77958173364&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0523.2009.01699.x
DO - 10.1111/j.1439-0523.2009.01699.x
M3 - Article
AN - SCOPUS:77958173364
VL - 129
SP - 448
EP - 453
JO - PLANT BREEDING
JF - PLANT BREEDING
SN - 0179-9541
IS - 4
ER -