Details
| Original language | English |
|---|---|
| Pages (from-to) | 2353-2360 |
| Number of pages | 8 |
| Journal | Nucleic acids research |
| Volume | 31 |
| Issue number | 9 |
| Publication status | Published - 1 May 2003 |
Abstract
ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F0F1-ATP synthase. To test this hypothesis, we have sequenced ymf39 from five protists with minimally derived mitochondrial genomes, the jakobids. In addition, we isolated the mitochondrial ATP synthase complex of the jakobid Seculamonas ecuadoriensis and determined the partial protein sequence of the 19-kDa subunit, the size expected for Ymf39. The obtained peptide sequence matches perfectly with a 3′-proximal region of the ymf39 gene of this organism, confirming that Ymf39 is indeed an ATP synthase subunit. Finally, we employed statistical tests to assess the significance of sequence similarity of Ymf39 proteins with each other, their nucleus-encoded functional counterparts, ATP4/ATP5F, from fungi and animals and α-proteobacterial ATP synthase b-subunits. This analysis provides clear evidence that ymf39 is an atpF homolog, while ATP4/ATP5F appears to be a highly diverged form of ymf39 that has migrated to the nucleus. We propose to designate ymf39 from now on atp4.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Nucleic acids research, Vol. 31, No. 9, 01.05.2003, p. 2353-2360.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b
AU - Burger, Gertraud
AU - Lang, B. Franz
AU - Braun, Hans Peter
AU - Marx, Stefanie
N1 - Funding information: We wish to thank T. Nerad (ATCC), who has isolated these important jakobid and malawimonad protists studied here. We also thank I. Plante for library construction and DNA sequencing, D. Dvzorno, I. Plante and Y. Zhu for DNA sequencing, M. Baumgaertner for peptide sequencing, S. Kannan for assistance in data analysis and E. O'Brien for critical reading of the manuscript. This work was supported by the `Deutsche Forschungsgemeinschaft', the `Fonds der Chemischen Industrie', and the `Canadian Institutes of Health Research' (CIHR, MSP-14226). G.B. is Canadian National Associate and B.F.L. is Imasco Fellow in the program of Evolutionary Biology of the Canadian Institute for Advanced Research (CIAR), whom we thank for salary and interaction support.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F0F1-ATP synthase. To test this hypothesis, we have sequenced ymf39 from five protists with minimally derived mitochondrial genomes, the jakobids. In addition, we isolated the mitochondrial ATP synthase complex of the jakobid Seculamonas ecuadoriensis and determined the partial protein sequence of the 19-kDa subunit, the size expected for Ymf39. The obtained peptide sequence matches perfectly with a 3′-proximal region of the ymf39 gene of this organism, confirming that Ymf39 is indeed an ATP synthase subunit. Finally, we employed statistical tests to assess the significance of sequence similarity of Ymf39 proteins with each other, their nucleus-encoded functional counterparts, ATP4/ATP5F, from fungi and animals and α-proteobacterial ATP synthase b-subunits. This analysis provides clear evidence that ymf39 is an atpF homolog, while ATP4/ATP5F appears to be a highly diverged form of ymf39 that has migrated to the nucleus. We propose to designate ymf39 from now on atp4.
AB - ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F0F1-ATP synthase. To test this hypothesis, we have sequenced ymf39 from five protists with minimally derived mitochondrial genomes, the jakobids. In addition, we isolated the mitochondrial ATP synthase complex of the jakobid Seculamonas ecuadoriensis and determined the partial protein sequence of the 19-kDa subunit, the size expected for Ymf39. The obtained peptide sequence matches perfectly with a 3′-proximal region of the ymf39 gene of this organism, confirming that Ymf39 is indeed an ATP synthase subunit. Finally, we employed statistical tests to assess the significance of sequence similarity of Ymf39 proteins with each other, their nucleus-encoded functional counterparts, ATP4/ATP5F, from fungi and animals and α-proteobacterial ATP synthase b-subunits. This analysis provides clear evidence that ymf39 is an atpF homolog, while ATP4/ATP5F appears to be a highly diverged form of ymf39 that has migrated to the nucleus. We propose to designate ymf39 from now on atp4.
UR - http://www.scopus.com/inward/record.url?scp=0038750910&partnerID=8YFLogxK
U2 - 10.1093/nar/gkg326
DO - 10.1093/nar/gkg326
M3 - Article
C2 - 12711680
AN - SCOPUS:0038750910
VL - 31
SP - 2353
EP - 2360
JO - Nucleic acids research
JF - Nucleic acids research
SN - 0305-1048
IS - 9
ER -