Details
| Original language | English |
|---|---|
| Pages (from-to) | 201-14 |
| Number of pages | 14 |
| Journal | Photosynthesis research |
| Volume | 106 |
| Issue number | 3 |
| Publication status | Published - Dec 2010 |
Abstract
Recent research on the photosynthetic mechanisms of plant species in the Chenopodiaceae family revealed that three species, including Bienertia sinuspersici, can carry out C(4) photosynthesis within individual photosynthetic cells, through the development of two cytoplasmic domains having dimorphic chloroplasts. These unusual single-cell C(4) species grow in semi-arid saline conditions and have semi-terete succulent leaves. The effects of salinity on growth and photosynthesis of B. sinuspersici were studied. The results show that NaCl is not required for development of the single-cell C(4) system. There is a large enhancement of growth in culture with 50-200 mM NaCl, while there is severe inhibition at 400 mM NaCl. With increasing salinity, the carbon isotope values (δ(13)C) of leaves increased from -17.3(o)/(oo) (C(4)-like) without NaCl to -14.6(o)/(oo) (C(4)) with 200 mM NaCl, possibly due to increased capture of CO(2) from the C(4) cycle by Rubisco and reduced leakiness. Compared to growth without NaCl, leaves of plants grown under saline conditions were much larger (~2 fold) and more succulent, and the leaf solute levels increased up to ~2000 mmol kg solvent(-1). Photosynthesis on an incident leaf area basis (CO(2) saturated rates, and carboxylation efficiency under limiting CO(2)) and stomatal conductance declined with increasing salinity. On a leaf area basis, there was some decline in Rubisco content with increasing salinity up to 200 mM NaCl, but there was a marked increase in the levels of pyruvate, Pi dikinase, and phosphoenolpyruvate carboxylase (possibly in response to sensitivity of these enzymes and C(4) cycle function to increasing salinity). The decline in photosynthesis on a leaf area basis was compensated for on a per leaf basis, up to 200 mM NaCl, by the increase in leaf size. The influence of salinity on plant development and the C(4) system in Bienertia is discussed.
Keywords
- Biomass, Blotting, Western, Carbon/metabolism, Carbon Dioxide/metabolism, Carbon Isotopes, Chenopodiaceae/cytology, Osmolar Concentration, Photosynthesis/drug effects, Plant Leaves/drug effects, Plant Roots/drug effects, Plant Shoots/drug effects, Plant Stomata/drug effects, Salinity, Sodium Chloride/pharmacology
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In: Photosynthesis research, Vol. 106, No. 3, 12.2010, p. 201-14.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The effects of salinity on photosynthesis and growth of the single-cell C4 species Bienertia sinuspersici (Chenopodiaceae)
AU - Leisner, Courtney P
AU - Cousins, Asaph B
AU - Offermann, Sascha
AU - Okita, Thomas W
AU - Edwards, Gerald E
N1 - Funding information: Acknowledgments This material is based upon work supported by the National Science Foundation under Grants IBN-0236959 and IBN-0641232. Seeds of Bienertia sinuspersici were kindly provided by Dr. Abdulrahman Alsirhan, Kuwait. We appreciate the advice of M.A. Evans on statistical analyses. We thank the Franceschi Microscopy and Imaging Center of Washington State University for use of facilities and for staff assistance, C. Cody for plant growth management, and E. Voznesenskaya, N. Kotey-eva, and O. Kiirats for helpful discussions during the research phase.
PY - 2010/12
Y1 - 2010/12
N2 - Recent research on the photosynthetic mechanisms of plant species in the Chenopodiaceae family revealed that three species, including Bienertia sinuspersici, can carry out C(4) photosynthesis within individual photosynthetic cells, through the development of two cytoplasmic domains having dimorphic chloroplasts. These unusual single-cell C(4) species grow in semi-arid saline conditions and have semi-terete succulent leaves. The effects of salinity on growth and photosynthesis of B. sinuspersici were studied. The results show that NaCl is not required for development of the single-cell C(4) system. There is a large enhancement of growth in culture with 50-200 mM NaCl, while there is severe inhibition at 400 mM NaCl. With increasing salinity, the carbon isotope values (δ(13)C) of leaves increased from -17.3(o)/(oo) (C(4)-like) without NaCl to -14.6(o)/(oo) (C(4)) with 200 mM NaCl, possibly due to increased capture of CO(2) from the C(4) cycle by Rubisco and reduced leakiness. Compared to growth without NaCl, leaves of plants grown under saline conditions were much larger (~2 fold) and more succulent, and the leaf solute levels increased up to ~2000 mmol kg solvent(-1). Photosynthesis on an incident leaf area basis (CO(2) saturated rates, and carboxylation efficiency under limiting CO(2)) and stomatal conductance declined with increasing salinity. On a leaf area basis, there was some decline in Rubisco content with increasing salinity up to 200 mM NaCl, but there was a marked increase in the levels of pyruvate, Pi dikinase, and phosphoenolpyruvate carboxylase (possibly in response to sensitivity of these enzymes and C(4) cycle function to increasing salinity). The decline in photosynthesis on a leaf area basis was compensated for on a per leaf basis, up to 200 mM NaCl, by the increase in leaf size. The influence of salinity on plant development and the C(4) system in Bienertia is discussed.
AB - Recent research on the photosynthetic mechanisms of plant species in the Chenopodiaceae family revealed that three species, including Bienertia sinuspersici, can carry out C(4) photosynthesis within individual photosynthetic cells, through the development of two cytoplasmic domains having dimorphic chloroplasts. These unusual single-cell C(4) species grow in semi-arid saline conditions and have semi-terete succulent leaves. The effects of salinity on growth and photosynthesis of B. sinuspersici were studied. The results show that NaCl is not required for development of the single-cell C(4) system. There is a large enhancement of growth in culture with 50-200 mM NaCl, while there is severe inhibition at 400 mM NaCl. With increasing salinity, the carbon isotope values (δ(13)C) of leaves increased from -17.3(o)/(oo) (C(4)-like) without NaCl to -14.6(o)/(oo) (C(4)) with 200 mM NaCl, possibly due to increased capture of CO(2) from the C(4) cycle by Rubisco and reduced leakiness. Compared to growth without NaCl, leaves of plants grown under saline conditions were much larger (~2 fold) and more succulent, and the leaf solute levels increased up to ~2000 mmol kg solvent(-1). Photosynthesis on an incident leaf area basis (CO(2) saturated rates, and carboxylation efficiency under limiting CO(2)) and stomatal conductance declined with increasing salinity. On a leaf area basis, there was some decline in Rubisco content with increasing salinity up to 200 mM NaCl, but there was a marked increase in the levels of pyruvate, Pi dikinase, and phosphoenolpyruvate carboxylase (possibly in response to sensitivity of these enzymes and C(4) cycle function to increasing salinity). The decline in photosynthesis on a leaf area basis was compensated for on a per leaf basis, up to 200 mM NaCl, by the increase in leaf size. The influence of salinity on plant development and the C(4) system in Bienertia is discussed.
KW - Biomass
KW - Blotting, Western
KW - Carbon/metabolism
KW - Carbon Dioxide/metabolism
KW - Carbon Isotopes
KW - Chenopodiaceae/cytology
KW - Osmolar Concentration
KW - Photosynthesis/drug effects
KW - Plant Leaves/drug effects
KW - Plant Roots/drug effects
KW - Plant Shoots/drug effects
KW - Plant Stomata/drug effects
KW - Salinity
KW - Sodium Chloride/pharmacology
U2 - 10.1007/s11120-010-9595-z
DO - 10.1007/s11120-010-9595-z
M3 - Article
C2 - 20838891
VL - 106
SP - 201
EP - 214
JO - Photosynthesis research
JF - Photosynthesis research
SN - 0166-8595
IS - 3
ER -