Details
| Original language | English |
|---|---|
| Pages (from-to) | 1880-1891 |
| Number of pages | 12 |
| Journal | BIOMATERIALS |
| Volume | 29 |
| Issue number | 12 |
| Publication status | Published - Apr 2008 |
Abstract
In this study we present the enzymatic and biological analysis of polysialic acid (polySia) based hydrogel in terms of its degradation and cytocompatibility. PolySia based hydrogel is completely degradable by endosialidase enzyme which may avoid second surgery after tissue recovery. Viability assay showed that soluble components of polySia hydrogel did not cause any toxic effect on cultured Schwann cells. Moreover, green fluorescence protein transfected neonatal and adult Schwann cells, neural stem cells and dorsal root ganglionic cells (unlabelled) were seeded on polySia hydrogel modified with poly-l-lysine (Pll), poly-l-ornithine-laminin (porn-laminin) or collagen. Water soluble tetrazolium salt assay revealed that modification of the hydrogel significantly improved cell adhesion and viability. These results infer that polySia based scaffolds in combination with cell adhesion molecules and cells genetically modified to express growth factors would potentially be promising alternative in reconstructive therapeutic strategies.
Keywords
- Dorsal root ganglia cells, Neural stem cells, Polysialic acid hydrogel, Schwann cells, Surface modification
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Ceramics and Composites
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Mechanics of Materials
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: BIOMATERIALS, Vol. 29, No. 12, 04.2008, p. 1880-1891.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells
AU - Haile, Yohannes
AU - Berski, Silke
AU - Dräger, Gerald
AU - Nobre, Andrè
AU - Stummeyer, Katharina
AU - Gerardy-Schahn, Rita
AU - Grothe, Claudia
N1 - Funding information: The authors wish to thank Dr. Haastert and Mrs.Wesemann for their help and technical advices. This work was supported by the German Research Foundation (DFG; FOR-548/1).
PY - 2008/4
Y1 - 2008/4
N2 - In this study we present the enzymatic and biological analysis of polysialic acid (polySia) based hydrogel in terms of its degradation and cytocompatibility. PolySia based hydrogel is completely degradable by endosialidase enzyme which may avoid second surgery after tissue recovery. Viability assay showed that soluble components of polySia hydrogel did not cause any toxic effect on cultured Schwann cells. Moreover, green fluorescence protein transfected neonatal and adult Schwann cells, neural stem cells and dorsal root ganglionic cells (unlabelled) were seeded on polySia hydrogel modified with poly-l-lysine (Pll), poly-l-ornithine-laminin (porn-laminin) or collagen. Water soluble tetrazolium salt assay revealed that modification of the hydrogel significantly improved cell adhesion and viability. These results infer that polySia based scaffolds in combination with cell adhesion molecules and cells genetically modified to express growth factors would potentially be promising alternative in reconstructive therapeutic strategies.
AB - In this study we present the enzymatic and biological analysis of polysialic acid (polySia) based hydrogel in terms of its degradation and cytocompatibility. PolySia based hydrogel is completely degradable by endosialidase enzyme which may avoid second surgery after tissue recovery. Viability assay showed that soluble components of polySia hydrogel did not cause any toxic effect on cultured Schwann cells. Moreover, green fluorescence protein transfected neonatal and adult Schwann cells, neural stem cells and dorsal root ganglionic cells (unlabelled) were seeded on polySia hydrogel modified with poly-l-lysine (Pll), poly-l-ornithine-laminin (porn-laminin) or collagen. Water soluble tetrazolium salt assay revealed that modification of the hydrogel significantly improved cell adhesion and viability. These results infer that polySia based scaffolds in combination with cell adhesion molecules and cells genetically modified to express growth factors would potentially be promising alternative in reconstructive therapeutic strategies.
KW - Dorsal root ganglia cells
KW - Neural stem cells
KW - Polysialic acid hydrogel
KW - Schwann cells
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=39749158440&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2007.12.030
DO - 10.1016/j.biomaterials.2007.12.030
M3 - Article
C2 - 18255143
AN - SCOPUS:39749158440
VL - 29
SP - 1880
EP - 1891
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 12
ER -