TY - JOUR

T1 - The effect of earthworm activity on soil bioporosity - Investigated with X-ray computed tomography and endoscopy

AU - Pagenkemper, Sebastian K.

AU - Athmann, Miriam

AU - Uteau, Daniel

AU - Kautz, Timo

AU - Peth, Stephan

AU - Horn, Rainer

N1 - Funding information: This study was supported by the German Research Foundation ( Deutsche Forschungsgemeinschaft – DFG) within the framework of the research unit DFG-FOR 1320. We further thank Heike Schneider and Alexandra Ley from the Juelich Research Station for carrying out the cultivation of the soil microcosms. Details of the field trials are documented under: www.for1320.uni-bonn.de . External researchers are free to join the running field trials.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Bioporosity in subsoils is strongly influenced by either deep rooting taproots or earthworms, which can affect important properties for plant growth provided by soils. The open question of this study was, in how far the combination of X-ray computed tomography (XRCT) and endoscopy may support the analysis of soil bioporosity and the effects induced by earthworm activity. The hypothesis were that earthworms can re-open, close and re-connect pores as well as change the biopore wall properties (by leaving coatings at the wall) and that with a combination of XRCT and endoscopy much more detailed information about those changes can be observed. Soil monoliths were collected from a Haplic Luvisol (developed from loess) at the experimental station Klein Altendorf (University of Bonn, Germany). The microcosms were then prepared under laboratory conditions in terms of temperature, soil temperature gradient, relative humidity, illumination, watering and fertilizer. The microcosms were examined before cultivation and incubation, and re-examined after one-month earthworm incubation (Lumbricus terrestris L.), three months wheat growth period and shoot harvesting. XRCT and endoscopy were used to qualitatively and quantitatively analyze the soil microcosms. After the incubation with earthworms, physical properties like porosity and accessible surface area of biopores have changed with respect to the original state. Coatings at the biopore walls resulted in smaller pore diameters of large biopores with a diameter >0.5. cm, while some of them have previously been pores with diameters <0.5. cm. Contrary to this, biopores with a diameter <0.5. cm were increased after earthworms, i.e. widened due earthworm passage. It was found that earthworms may have disconnected lateral pores that reach into the bulk soil from the vertical biopores by pore wall coatings. After incubation, biopores lined with fresh earthworm coatings in the monolith increased from 30% to 80%, which may indicate that earthworms potentially have a considerable influence on biopore properties and therefore the physical, chemical and microbiological environment which roots are exposed to in biopores.

AB - Bioporosity in subsoils is strongly influenced by either deep rooting taproots or earthworms, which can affect important properties for plant growth provided by soils. The open question of this study was, in how far the combination of X-ray computed tomography (XRCT) and endoscopy may support the analysis of soil bioporosity and the effects induced by earthworm activity. The hypothesis were that earthworms can re-open, close and re-connect pores as well as change the biopore wall properties (by leaving coatings at the wall) and that with a combination of XRCT and endoscopy much more detailed information about those changes can be observed. Soil monoliths were collected from a Haplic Luvisol (developed from loess) at the experimental station Klein Altendorf (University of Bonn, Germany). The microcosms were then prepared under laboratory conditions in terms of temperature, soil temperature gradient, relative humidity, illumination, watering and fertilizer. The microcosms were examined before cultivation and incubation, and re-examined after one-month earthworm incubation (Lumbricus terrestris L.), three months wheat growth period and shoot harvesting. XRCT and endoscopy were used to qualitatively and quantitatively analyze the soil microcosms. After the incubation with earthworms, physical properties like porosity and accessible surface area of biopores have changed with respect to the original state. Coatings at the biopore walls resulted in smaller pore diameters of large biopores with a diameter >0.5. cm, while some of them have previously been pores with diameters <0.5. cm. Contrary to this, biopores with a diameter <0.5. cm were increased after earthworms, i.e. widened due earthworm passage. It was found that earthworms may have disconnected lateral pores that reach into the bulk soil from the vertical biopores by pore wall coatings. After incubation, biopores lined with fresh earthworm coatings in the monolith increased from 30% to 80%, which may indicate that earthworms potentially have a considerable influence on biopore properties and therefore the physical, chemical and microbiological environment which roots are exposed to in biopores.

KW - Drilosphere

KW - Dynamic Soil structure

KW - Microcosm

KW - Non-invasive methods

KW - Pore wall properties

UR - http://www.scopus.com/inward/record.url?scp=84911389611&partnerID=8YFLogxK

U2 - 10.1016/j.still.2014.05.007

DO - 10.1016/j.still.2014.05.007

M3 - Article

AN - SCOPUS:84911389611

VL - 146

SP - 79

EP - 88

JO - Soil and Tillage Research

JF - Soil and Tillage Research

SN - 0167-1987

IS - PA

ER -