Details
| Original language | English |
|---|---|
| Pages (from-to) | 473-485 |
| Number of pages | 13 |
| Journal | ISME Journal |
| Volume | 5 |
| Issue number | 3 |
| Publication status | Published - Mar 2011 |
| Externally published | Yes |
Abstract
2-Methyl-4-chlorophenoxyacetic acid (MCPA) is a widely used phenoxyalkanoic acid (PAA) herbicide. Earthworms represent the dominant macrofauna and enhance microbial activities in many soils. Thus, the effect of the model earthworm Aporrectodea caliginosa (Oligochaeta, Lumbricidae) on microbial MCPA degradation was assessed in soil columns with agricultural soil. MCPA degradation was quicker in soil with earthworms than without earthworms. Quantitative PCR was inhibition-corrected per nucleic acid extract and indicated that copy numbers of tfdA-like and cadA genes (both encoding oxygenases initiating aerobic PAA degradation) in soil with earthworms were up to three and four times higher than without earthworms, respectively. tfdA-like and 16S rRNA gene transcript copy numbers in soil with earthworms were two and six times higher than without earthworms, respectively. Most probable numbers (MPNs) of MCPA degraders approximated 4 × 10 5 g dw -1 in soil before incubation and in soil treated without earthworms, whereas MPNs of earthworm-treated soils were approximately 150 × higher. The aerobic capacity of soil to degrade MCPA was higher in earthworm-treated soils than in earthworm-untreated soils. Burrow walls and 0-5 cm depth bulk soil displayed higher capacities to degrade MCPA than did soil from 5-10 cm depth bulk soil, expression of tfdA-like genes in burrow walls was five times higher than in bulk soil and MCPA degraders were abundant in burrow walls (MPNs of 5 × 10 7 g dw -1). The collective data indicate that earthworms stimulate abundance and activity of MCPA degraders endogenous to soil by their burrowing activities and might thus be advantageous for enhancing PAA degradation in soil.
Keywords
- bioconcentration, cadA, drilosphere, gene expression, mRNA, quantitative PCR, relative gene frequency ratio, tfdA-like
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
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In: ISME Journal, Vol. 5, No. 3, 03.2011, p. 473-485.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The earthworm Aporrectodea caliginosa stimulates abundance and activity of phenoxyalkanoic acid herbicide degraders
AU - Liu, Ya Jun
AU - Zaprasis, Adrienne
AU - Liu, Shuang Jiang
AU - Drake, Harold L.
AU - Horn, Marcus A.
N1 - Funding Information: We are grateful to Stephan Schulz, Michael Schloter and Jean Charles Munch for provision of soil samples; Klaus-Holger Knorr and Julia Beer for help with scintillation counting; and Martina Gocke, Ilse Thaufelder and Yakov Kuzyakov for support with the analysis of non-extractable residues of MCPA. Support for this study was provided by the Deutsche Forschungsgemeinschaft (DFG Grant HO4020/1-1, DFG Priority Program 1315 ‘Biogeochemical interfaces in soil’), by the University of Bayreuth and by grants from the Chinese Academy of Sciences (KSCX2-SW-G-052) and the German Academic Exchange Service (DAAD) to Y-JL. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/3
Y1 - 2011/3
N2 - 2-Methyl-4-chlorophenoxyacetic acid (MCPA) is a widely used phenoxyalkanoic acid (PAA) herbicide. Earthworms represent the dominant macrofauna and enhance microbial activities in many soils. Thus, the effect of the model earthworm Aporrectodea caliginosa (Oligochaeta, Lumbricidae) on microbial MCPA degradation was assessed in soil columns with agricultural soil. MCPA degradation was quicker in soil with earthworms than without earthworms. Quantitative PCR was inhibition-corrected per nucleic acid extract and indicated that copy numbers of tfdA-like and cadA genes (both encoding oxygenases initiating aerobic PAA degradation) in soil with earthworms were up to three and four times higher than without earthworms, respectively. tfdA-like and 16S rRNA gene transcript copy numbers in soil with earthworms were two and six times higher than without earthworms, respectively. Most probable numbers (MPNs) of MCPA degraders approximated 4 × 10 5 g dw -1 in soil before incubation and in soil treated without earthworms, whereas MPNs of earthworm-treated soils were approximately 150 × higher. The aerobic capacity of soil to degrade MCPA was higher in earthworm-treated soils than in earthworm-untreated soils. Burrow walls and 0-5 cm depth bulk soil displayed higher capacities to degrade MCPA than did soil from 5-10 cm depth bulk soil, expression of tfdA-like genes in burrow walls was five times higher than in bulk soil and MCPA degraders were abundant in burrow walls (MPNs of 5 × 10 7 g dw -1). The collective data indicate that earthworms stimulate abundance and activity of MCPA degraders endogenous to soil by their burrowing activities and might thus be advantageous for enhancing PAA degradation in soil.
AB - 2-Methyl-4-chlorophenoxyacetic acid (MCPA) is a widely used phenoxyalkanoic acid (PAA) herbicide. Earthworms represent the dominant macrofauna and enhance microbial activities in many soils. Thus, the effect of the model earthworm Aporrectodea caliginosa (Oligochaeta, Lumbricidae) on microbial MCPA degradation was assessed in soil columns with agricultural soil. MCPA degradation was quicker in soil with earthworms than without earthworms. Quantitative PCR was inhibition-corrected per nucleic acid extract and indicated that copy numbers of tfdA-like and cadA genes (both encoding oxygenases initiating aerobic PAA degradation) in soil with earthworms were up to three and four times higher than without earthworms, respectively. tfdA-like and 16S rRNA gene transcript copy numbers in soil with earthworms were two and six times higher than without earthworms, respectively. Most probable numbers (MPNs) of MCPA degraders approximated 4 × 10 5 g dw -1 in soil before incubation and in soil treated without earthworms, whereas MPNs of earthworm-treated soils were approximately 150 × higher. The aerobic capacity of soil to degrade MCPA was higher in earthworm-treated soils than in earthworm-untreated soils. Burrow walls and 0-5 cm depth bulk soil displayed higher capacities to degrade MCPA than did soil from 5-10 cm depth bulk soil, expression of tfdA-like genes in burrow walls was five times higher than in bulk soil and MCPA degraders were abundant in burrow walls (MPNs of 5 × 10 7 g dw -1). The collective data indicate that earthworms stimulate abundance and activity of MCPA degraders endogenous to soil by their burrowing activities and might thus be advantageous for enhancing PAA degradation in soil.
KW - bioconcentration
KW - cadA
KW - drilosphere
KW - gene expression
KW - mRNA
KW - quantitative PCR
KW - relative gene frequency ratio
KW - tfdA-like
UR - http://www.scopus.com/inward/record.url?scp=79951811482&partnerID=8YFLogxK
U2 - 10.1038/ismej.2010.140
DO - 10.1038/ismej.2010.140
M3 - Article
C2 - 20740027
AN - SCOPUS:79951811482
VL - 5
SP - 473
EP - 485
JO - ISME Journal
JF - ISME Journal
SN - 1751-7362
IS - 3
ER -