Details
| Original language | English |
|---|---|
| Article number | 108353 |
| Journal | Soil Biology and Biochemistry |
| Volume | 160 |
| Early online date | 4 Jul 2021 |
| Publication status | Published - Sept 2021 |
| Externally published | Yes |
Abstract
The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined by the substrate sources and availability, yet evidence provided by quantification of their respective gross activities in soil is lacking. Here, we conducted a microcosm study with agricultural soil with high (50 μg N g −1) and low (5 μg N g −1) ammonium application, and quantified gross nitrification rates using 15N-tracers. AOA and AOB activities were distinguished using the bacterial inhibitor 1-octyne and acetylene, which inhibits both AOA and AOB. Under low ammonium supply, AOA and AOB contributed equally to gross ammonia oxidation, but AOB outcompeted AOA under higher ammonium supply. These results provide the first direct evidence that substrate availability affects the relative contribution of AOA and AOB to gross nitrification.
Keywords
- 1-Octyne, Acetylene, Ammonia oxidation, N-tracing
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
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In: Soil Biology and Biochemistry, Vol. 160, 108353, 09.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The contribution of ammonia-oxidizing archaea and bacteria to gross nitrification under different substrate availability
AU - Rütting, Tobias
AU - Schleusner, Philipp
AU - Hink, Linda
AU - Prosser, James I.
N1 - Funding Information: The work was supported by the European Union ( Marie Curie ITN NORA , FP7- 316472 ). This publication is part of the Swedish strategic research area ‘‘Biodiversity and Ecosystem services in a Changing Climate - BECC’’ ( www.becc.lu.se/ ).
PY - 2021/9
Y1 - 2021/9
N2 - The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined by the substrate sources and availability, yet evidence provided by quantification of their respective gross activities in soil is lacking. Here, we conducted a microcosm study with agricultural soil with high (50 μg N g −1) and low (5 μg N g −1) ammonium application, and quantified gross nitrification rates using 15N-tracers. AOA and AOB activities were distinguished using the bacterial inhibitor 1-octyne and acetylene, which inhibits both AOA and AOB. Under low ammonium supply, AOA and AOB contributed equally to gross ammonia oxidation, but AOB outcompeted AOA under higher ammonium supply. These results provide the first direct evidence that substrate availability affects the relative contribution of AOA and AOB to gross nitrification.
AB - The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined by the substrate sources and availability, yet evidence provided by quantification of their respective gross activities in soil is lacking. Here, we conducted a microcosm study with agricultural soil with high (50 μg N g −1) and low (5 μg N g −1) ammonium application, and quantified gross nitrification rates using 15N-tracers. AOA and AOB activities were distinguished using the bacterial inhibitor 1-octyne and acetylene, which inhibits both AOA and AOB. Under low ammonium supply, AOA and AOB contributed equally to gross ammonia oxidation, but AOB outcompeted AOA under higher ammonium supply. These results provide the first direct evidence that substrate availability affects the relative contribution of AOA and AOB to gross nitrification.
KW - 1-Octyne
KW - Acetylene
KW - Ammonia oxidation
KW - N-tracing
UR - http://www.scopus.com/inward/record.url?scp=85109210657&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2021.108353
DO - 10.1016/j.soilbio.2021.108353
M3 - Article
VL - 160
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
M1 - 108353
ER -