Details
| Original language | English |
|---|---|
| Pages (from-to) | 4926-4929 |
| Number of pages | 4 |
| Journal | Angewandte Chemie |
| Volume | 46 |
| Issue number | 26 |
| Publication status | Published - 18 Jul 2007 |
| Externally published | Yes |
Abstract
(Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
Keywords
- Enzymes, Isotopic labeling, Kinetic isotope effects, Reaction mechanisms
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
- General Chemistry
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In: Angewandte Chemie , Vol. 46, No. 26, 18.07.2007, p. 4926-4929.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coli
AU - Wong, Ursula
AU - Cox, Russell J.
PY - 2007/7/18
Y1 - 2007/7/18
N2 - (Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
AB - (Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
KW - Enzymes
KW - Isotopic labeling
KW - Kinetic isotope effects
KW - Reaction mechanisms
UR - http://www.scopus.com/inward/record.url?scp=34447344507&partnerID=8YFLogxK
U2 - 10.1002/anie.200700647
DO - 10.1002/anie.200700647
M3 - Article
C2 - 17516600
AN - SCOPUS:34447344507
VL - 46
SP - 4926
EP - 4929
JO - Angewandte Chemie
JF - Angewandte Chemie
SN - 1433-7851
IS - 26
ER -