Details
| Original language | English |
|---|---|
| Pages (from-to) | 523-530 |
| Number of pages | 8 |
| Journal | BBA - General Subjects |
| Volume | 883 |
| Issue number | 3 |
| Publication status | Published - 1 Oct 1986 |
| Externally published | Yes |
Abstract
A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
Keywords
- (A. repens), C Aldehyde, Enzyme inactivation, Enzyme selectivity, Fatty acid hydroperoxide, Hexenal, Lipoxygenase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: BBA - General Subjects, Vol. 883, No. 3, 01.10.1986, p. 523-530.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The C6-aldehyde-forming system in Agropyron repens
AU - Berger, Ralf G.
AU - Kler, Adolf
AU - Drawert, Friedrich
N1 - Funding information: The project was supported by the BMFT (Federal Ministry of Science and Technology), Bonn. S. Nitz and H. Pivernetza ssistedin mass spectrometryW. e are gratefult o G. Leupold for supplyingc apillaryc olumns.
PY - 1986/10/1
Y1 - 1986/10/1
N2 - A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
AB - A vigorous formation of the volatile C6 compounds typical of plant lipoxygenase/hydroperoxide lyase systems (n-hexanal and hexenals) was observed in mechanically disrupted tissue of quick grass (Agropyron repens). Evidence for the precursor properties of linolenic acid and for the primary main cleavage product (Z)-3-hexenal was obtained from time-course studies. Both the lipoxygenase and the hydroperoxide lyase activities occur in a pellet sedimenting at 15 000 × g. The relative proportions of 13- to 9-hydroperoxi-(E,Z)-10,12-octadecadienoic acids were 81 to 19 under the conditions used. The enantiomeric composition of the 13-hydroperoxide, the sole substrate for the lyase, was approx. 74 to 26 (13S to 13R) and remained unchanged during prolonged incubation. The contribution of lytic activities to the formation of 1-penten-3-ol is suggested. The limited activity of the hexenal-forming system was neither caused by the carbonyls themselves nor by a hydroperoxide deficiency, and the inactivation followed third-order reaction kinetics.
KW - (A. repens)
KW - C Aldehyde
KW - Enzyme inactivation
KW - Enzyme selectivity
KW - Fatty acid hydroperoxide
KW - Hexenal
KW - Lipoxygenase
UR - http://www.scopus.com/inward/record.url?scp=0000684930&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(86)90293-X
DO - 10.1016/0304-4165(86)90293-X
M3 - Article
AN - SCOPUS:0000684930
VL - 883
SP - 523
EP - 530
JO - BBA - General Subjects
JF - BBA - General Subjects
SN - 0304-4165
IS - 3
ER -