Details
| Original language | English |
|---|---|
| Pages (from-to) | 278-290 |
| Number of pages | 13 |
| Journal | Molecular Plant Pathology |
| Volume | 23 |
| Issue number | 2 |
| Early online date | 23 Nov 2021 |
| Publication status | Published - 9 Jan 2022 |
Abstract
High-yielding and stress-resistant crops are essential to ensure future food supply. Barley is an important crop to feed livestock and to produce malt, but the annual yield is threatened by pathogen infections. Pathogens can trigger an altered sugar partitioning in the host plant, which possibly leads to an advantage for the pathogen. Hampering these processes represents a promising strategy to potentially increase resistance. We analysed the response of the barley monosaccharide transporter HvSTP13 towards biotic stress and its potential use for plant protection. The expression of HvSTP13 increased on bacterial and fungal pathogen-associated molecular pattern (PAMP) application, suggesting a PAMP-triggered signalling that converged on the transcriptional induction of the gene. Promoter studies indicate a region that is probably targeted by transcription factors downstream of PAMP-triggered immunity pathways. We confirmed that the nonfunctional HvSTP13GR variant confers resistance against an economically relevant biotrophic rust fungus in barley. Our experimental setup provides basal prerequisites to further decode the role of HvSTP13 in response to biological stress. Moreover, in line with other studies, our experiments indicate that the alteration of sugar partitioning pathways, in a host–pathogen interaction, is a promising approach to achieve broad and durable resistance in plants.
Keywords
- HvSTP13, PAMP, barley, fungi, resistance, sugar
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Soil Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Molecular Plant Pathology, Vol. 23, No. 2, 09.01.2022, p. 278-290.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The barley HvSTP13GR mutant triggers resistance against biotrophic fungi
AU - Skoppek, Caroline Ines
AU - Punt, Wilko
AU - Heinrichs, Marleen
AU - Ordon, Frank
AU - Wehner, Gwendolin
AU - Boch, Jens
AU - Streubel, Jana
N1 - Funding Information: We thank Anna Marthe, Christine Hoppe, and Ilona Renneberg for technical assistance and Sebastian Becker and Annekatrin Richter for helpful discussions on the manuscript. We are grateful to Claude Bragard and Ralf Koebnik for providing Xth UPB820 and Alain Tissier for providing yeast MoClo plasmids. This work was supported by university core funding only. R
PY - 2022/1/9
Y1 - 2022/1/9
N2 - High-yielding and stress-resistant crops are essential to ensure future food supply. Barley is an important crop to feed livestock and to produce malt, but the annual yield is threatened by pathogen infections. Pathogens can trigger an altered sugar partitioning in the host plant, which possibly leads to an advantage for the pathogen. Hampering these processes represents a promising strategy to potentially increase resistance. We analysed the response of the barley monosaccharide transporter HvSTP13 towards biotic stress and its potential use for plant protection. The expression of HvSTP13 increased on bacterial and fungal pathogen-associated molecular pattern (PAMP) application, suggesting a PAMP-triggered signalling that converged on the transcriptional induction of the gene. Promoter studies indicate a region that is probably targeted by transcription factors downstream of PAMP-triggered immunity pathways. We confirmed that the nonfunctional HvSTP13GR variant confers resistance against an economically relevant biotrophic rust fungus in barley. Our experimental setup provides basal prerequisites to further decode the role of HvSTP13 in response to biological stress. Moreover, in line with other studies, our experiments indicate that the alteration of sugar partitioning pathways, in a host–pathogen interaction, is a promising approach to achieve broad and durable resistance in plants.
AB - High-yielding and stress-resistant crops are essential to ensure future food supply. Barley is an important crop to feed livestock and to produce malt, but the annual yield is threatened by pathogen infections. Pathogens can trigger an altered sugar partitioning in the host plant, which possibly leads to an advantage for the pathogen. Hampering these processes represents a promising strategy to potentially increase resistance. We analysed the response of the barley monosaccharide transporter HvSTP13 towards biotic stress and its potential use for plant protection. The expression of HvSTP13 increased on bacterial and fungal pathogen-associated molecular pattern (PAMP) application, suggesting a PAMP-triggered signalling that converged on the transcriptional induction of the gene. Promoter studies indicate a region that is probably targeted by transcription factors downstream of PAMP-triggered immunity pathways. We confirmed that the nonfunctional HvSTP13GR variant confers resistance against an economically relevant biotrophic rust fungus in barley. Our experimental setup provides basal prerequisites to further decode the role of HvSTP13 in response to biological stress. Moreover, in line with other studies, our experiments indicate that the alteration of sugar partitioning pathways, in a host–pathogen interaction, is a promising approach to achieve broad and durable resistance in plants.
KW - HvSTP13
KW - PAMP
KW - barley
KW - fungi
KW - resistance
KW - sugar
UR - http://www.scopus.com/inward/record.url?scp=85119657824&partnerID=8YFLogxK
U2 - 10.1111/mpp.13161
DO - 10.1111/mpp.13161
M3 - Article
VL - 23
SP - 278
EP - 290
JO - Molecular Plant Pathology
JF - Molecular Plant Pathology
SN - 1464-6722
IS - 2
ER -