The automation of immun-FIA-systems

Research output: Contribution to journalArticleResearchpeer review

Authors

  • B. Hitzmann
  • A. Löhn
  • M. Reinecke
  • B. Schulze
  • T. Scheper

External Research Organisations

  • University of Münster
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Details

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalAnalytica chimica acta
Volume313
Issue number1-2
Publication statusPublished - 20 Sept 1995
Externally publishedYes

Abstract

In this contribution an automation system for the control, measurement uptake, evaluation and visualization is presented as well as its application for two different flow-injection immunoassays. Both systems are based on the principles of flow-injection analysis and were developed for a reliable, fast monitoring of relevant proteins in animal cell cultivation processes. One system uses a homogeneous assay with turbidimetric detection, the other a heterogeneous assay with an immobilized immunocomponent applying fluorescence detection. The on-line application during a mammalian cell cultivation as well as off-line measurements of medium samples are presented. The results obtained from on-line measurements are compared to ELISA results. The automation of the analysis systems with respect to its flexible control as well as the reliable evaluation of the measurement signals -even if they are severely distorted -are discussed in detail.

Keywords

    Automation, Bioprocess monitoring, Flow injection, Immunoassay, Reliable evaluation

ASJC Scopus subject areas

Cite this

The automation of immun-FIA-systems. / Hitzmann, B.; Löhn, A.; Reinecke, M. et al.
In: Analytica chimica acta, Vol. 313, No. 1-2, 20.09.1995, p. 55-62.

Research output: Contribution to journalArticleResearchpeer review

Hitzmann, B, Löhn, A, Reinecke, M, Schulze, B & Scheper, T 1995, 'The automation of immun-FIA-systems', Analytica chimica acta, vol. 313, no. 1-2, pp. 55-62. https://doi.org/10.1016/0003-2670(95)00173-W
Hitzmann, B., Löhn, A., Reinecke, M., Schulze, B., & Scheper, T. (1995). The automation of immun-FIA-systems. Analytica chimica acta, 313(1-2), 55-62. https://doi.org/10.1016/0003-2670(95)00173-W
Hitzmann B, Löhn A, Reinecke M, Schulze B, Scheper T. The automation of immun-FIA-systems. Analytica chimica acta. 1995 Sept 20;313(1-2):55-62. doi: 10.1016/0003-2670(95)00173-W
Hitzmann, B. ; Löhn, A. ; Reinecke, M. et al. / The automation of immun-FIA-systems. In: Analytica chimica acta. 1995 ; Vol. 313, No. 1-2. pp. 55-62.
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