Details
| Original language | English |
|---|---|
| Pages (from-to) | 607-611 |
| Number of pages | 5 |
| Journal | Enzyme and microbial technology |
| Volume | 39 |
| Issue number | 4 |
| Publication status | Published - 24 Jan 2006 |
Abstract
We recently developed a pseudo-enantiomeric reaction which was monitored on-line via 2D-fluorescence spectroscopy in different reaction media. During enzymatic reactions, conclusions about the converted substrates and the enantioselectivity could be drawn immediately, using two different fluorescence spectroscopic detectable substrates, l-phenylalanine-7-amido-4-methylcoumarine and d-phenylalanine-7-amido-4-trifluoromethylcoumarine. Now, due to the enlargement of one of the coumarin substrates via salt formation, it was possible to influence the enzymatyic enantioselectivity in toluene and to monitor the impact of the bulky counterion on-line.
Keywords
- 2D-fluorescencespectroscopy, Coumarines, Esterase, Improving enantioselectivity, On-line monitoring, Organic solvents, Substrate salts
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Enzyme and microbial technology, Vol. 39, No. 4, 24.01.2006, p. 607-611.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The application of two-dimensional fluorescence spectroscopy for the on-line evaluation of modified enzymatic enantioselectivities in organic solvents by forming substrate salts
AU - Knüttel, Torsten
AU - Meyer, Hartmut
AU - Scheper, Thomas
N1 - Funding information: The authors thank the Deutsche Forschungsgemeinschaft (DFG, as part of the Graduiertenkolleg) for financial support.
PY - 2006/1/24
Y1 - 2006/1/24
N2 - We recently developed a pseudo-enantiomeric reaction which was monitored on-line via 2D-fluorescence spectroscopy in different reaction media. During enzymatic reactions, conclusions about the converted substrates and the enantioselectivity could be drawn immediately, using two different fluorescence spectroscopic detectable substrates, l-phenylalanine-7-amido-4-methylcoumarine and d-phenylalanine-7-amido-4-trifluoromethylcoumarine. Now, due to the enlargement of one of the coumarin substrates via salt formation, it was possible to influence the enzymatyic enantioselectivity in toluene and to monitor the impact of the bulky counterion on-line.
AB - We recently developed a pseudo-enantiomeric reaction which was monitored on-line via 2D-fluorescence spectroscopy in different reaction media. During enzymatic reactions, conclusions about the converted substrates and the enantioselectivity could be drawn immediately, using two different fluorescence spectroscopic detectable substrates, l-phenylalanine-7-amido-4-methylcoumarine and d-phenylalanine-7-amido-4-trifluoromethylcoumarine. Now, due to the enlargement of one of the coumarin substrates via salt formation, it was possible to influence the enzymatyic enantioselectivity in toluene and to monitor the impact of the bulky counterion on-line.
KW - 2D-fluorescencespectroscopy
KW - Coumarines
KW - Esterase
KW - Improving enantioselectivity
KW - On-line monitoring
KW - Organic solvents
KW - Substrate salts
UR - http://www.scopus.com/inward/record.url?scp=33745195710&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2005.11.026
DO - 10.1016/j.enzmictec.2005.11.026
M3 - Article
AN - SCOPUS:33745195710
VL - 39
SP - 607
EP - 611
JO - Enzyme and microbial technology
JF - Enzyme and microbial technology
SN - 0141-0229
IS - 4
ER -