Details
| Original language | English |
|---|---|
| Article number | 003902 |
| Pages (from-to) | 1192-1202 |
| Number of pages | 11 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 70 |
| Issue number | 2 |
| Publication status | Published - Feb 2020 |
| Externally published | Yes |
Abstract
A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).
Keywords
- Chloroflexi, Chukotka hot spring, Laser bioprinting, LEMS, Microbial dark matter, Tepidiforma, RNA, Ribosomal, 16S/genetics, Phospholipids/chemistry, Phylogeny, Sequence Analysis, DNA, Russia, Chloroflexi/classification, Hot Springs/microbiology, Carotenoids/chemistry, Arctic Regions, Base Composition, Bacterial Typing Techniques, Fatty Acids/chemistry, DNA, Bacterial/genetics
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Immunology and Microbiology(all)
- Microbiology
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In: International Journal of Systematic and Evolutionary Microbiology, Vol. 70, No. 2, 003902, 02.2020, p. 1192-1202.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14
AU - Kochetkova, Tatiana V.
AU - Zayulina, Kseniya S.
AU - Zhigarkov, Vyacheslav S.
AU - Minaev, Nikita V.
AU - Chichkov, Boris N.
AU - Novikov, Andrei A.
AU - Toshchakov, Stepan V.
AU - Elcheninov, Alexander G.
AU - Kublanov, Ilya V.
N1 - Funding information: This work of T. V. K., K. S. Z. and A. G. E. was supported by the Russian Foundation for Basic Research (grant number 17-04-01578). The Ministry of Science and Higher Education of Russia supported the work of IVK and SVT. N. V. M. was granted a Scholarship of the President of the Russian Federation SP-2728.2019.4. Chemotaxonomic studies (A. A. N.) were supported by the President of Russia (grant ???3801.2018.4; agreement 075-15-2019-1025).
PY - 2020/2
Y1 - 2020/2
N2 - A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).
AB - A novel aerobic moderately thermophilic bacterium, strain 3753OT, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753OT grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO3) as the electron donor. The major cellular fatty acid was branched C19:0. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phos-pholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753OT was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753OT within the previously uncultivated lineage OLB14 in the phylum Chloroflexi. Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species Tepidiforma bonchosmolovskayae gen. nov., sp. nov., within the Tepidiformaceae fam. nov., the Tepidiformales ord. nov. and the Tepidiformia classis nov. are proposed. The type strain of Tepidiforma bonchosmolovskayae is 3753OT (=VKM B-3389T=KTCT 72284T).
KW - Chloroflexi
KW - Chukotka hot spring
KW - Laser bioprinting
KW - LEMS
KW - Microbial dark matter
KW - Tepidiforma
KW - RNA, Ribosomal, 16S/genetics
KW - Phospholipids/chemistry
KW - Phylogeny
KW - Sequence Analysis, DNA
KW - Russia
KW - Chloroflexi/classification
KW - Hot Springs/microbiology
KW - Carotenoids/chemistry
KW - Arctic Regions
KW - Base Composition
KW - Bacterial Typing Techniques
KW - Fatty Acids/chemistry
KW - DNA, Bacterial/genetics
UR - http://www.scopus.com/inward/record.url?scp=85081139572&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003902
DO - 10.1099/ijsem.0.003902
M3 - Article
C2 - 31769750
AN - SCOPUS:85081139572
VL - 70
SP - 1192
EP - 1202
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 2
M1 - 003902
ER -