Details
| Original language | English |
|---|---|
| Pages (from-to) | 1035-1041 |
| Number of pages | 7 |
| Journal | Artificial Organs |
| Volume | 43 |
| Issue number | 10 |
| Early online date | 18 Jun 2019 |
| Publication status | Published - 1 Oct 2019 |
| Externally published | Yes |
Abstract
Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.
Keywords
- 3D cell culture, 3D-printing, bioreactor, optogenetics, recellularization
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Artificial Organs, Vol. 43, No. 10, 01.10.2019, p. 1035-1041.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Teburu—Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models
AU - Daneshgar, Assal
AU - Tang, Peter
AU - Remde, Christopher
AU - Lommel, Michael
AU - Moosburner, Simon
AU - Kertzscher, Ulrich
AU - Klein, Oliver
AU - Weinhart, Marie
AU - Pratschke, Johann
AU - Sauer, Igor M.
AU - Hillebrandt, Karl H.
N1 - Funding information: The authors thank Prof. Dr.-Ing. Klaus Affeld from the Biofluid Machanics Laboratory and the Institute for Imaging Science and Computational Modeling in Cardiovascular Medicine at Charité – Universitätsmedizin Berlin for his kind cooperation regarding Teburu's fluid dynamics and flow simulation. Furthermore, the authors thank the Einstein Center for Regenerative Therapies for funding this project.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.
AB - Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.
KW - 3D cell culture
KW - 3D-printing
KW - bioreactor
KW - optogenetics
KW - recellularization
UR - http://www.scopus.com/inward/record.url?scp=85069856159&partnerID=8YFLogxK
U2 - 10.1111/aor.13518
DO - 10.1111/aor.13518
M3 - Article
C2 - 31211867
AN - SCOPUS:85069856159
VL - 43
SP - 1035
EP - 1041
JO - Artificial Organs
JF - Artificial Organs
SN - 0160-564X
IS - 10
ER -