Details
| Original language | English |
|---|---|
| Pages (from-to) | 75-82 |
| Number of pages | 8 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 85 |
| Issue number | 1 |
| Publication status | Published - 4 Apr 2006 |
Abstract
In order to optimize shoot regeneration in Kalanchoë blossfeldiana, leaf and internode explants of seven cultivars including one inter-specific were studied. The effects of various combinations of α-naphthalene acetic acid (NAA) (0, 0.57 M) and thidiazuron (TDZ) (0, 0.45, 4.5, 22.5, 67.5 μM) on MS medium were examined. In all cultivars shoot regeneration frequency and number of shoots per explant were enhanced by increasing TDZ concentration. Supplementing the media with NAA did not improve shoot regeneration. Maximum regeneration frequency and optimum concentration of TDZ for shoot regeneration depended significantly on the cultivar. Internode explants, but not leaf explants, of some cultivars, were able to produce adventitious shoots without treatment with growth regulator.
Keywords
- in vitro cultivation, Internode explant, Leaf explant, Plant age, Shoot elongation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 85, No. 1, 04.04.2006, p. 75-82.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin
AU - Sanikhani, Mohsen
AU - Frello, Stefan
AU - Serek, Margrethe
N1 - Funding Information: This investigation was supported by the Iranian Ministry of Science, Research & Technology. We thank Knud Jepsen A/S, Dambrovej 53, Hinn-erup, Denmark, for providing us with plants for this investigation.
PY - 2006/4/4
Y1 - 2006/4/4
N2 - In order to optimize shoot regeneration in Kalanchoë blossfeldiana, leaf and internode explants of seven cultivars including one inter-specific were studied. The effects of various combinations of α-naphthalene acetic acid (NAA) (0, 0.57 M) and thidiazuron (TDZ) (0, 0.45, 4.5, 22.5, 67.5 μM) on MS medium were examined. In all cultivars shoot regeneration frequency and number of shoots per explant were enhanced by increasing TDZ concentration. Supplementing the media with NAA did not improve shoot regeneration. Maximum regeneration frequency and optimum concentration of TDZ for shoot regeneration depended significantly on the cultivar. Internode explants, but not leaf explants, of some cultivars, were able to produce adventitious shoots without treatment with growth regulator.
AB - In order to optimize shoot regeneration in Kalanchoë blossfeldiana, leaf and internode explants of seven cultivars including one inter-specific were studied. The effects of various combinations of α-naphthalene acetic acid (NAA) (0, 0.57 M) and thidiazuron (TDZ) (0, 0.45, 4.5, 22.5, 67.5 μM) on MS medium were examined. In all cultivars shoot regeneration frequency and number of shoots per explant were enhanced by increasing TDZ concentration. Supplementing the media with NAA did not improve shoot regeneration. Maximum regeneration frequency and optimum concentration of TDZ for shoot regeneration depended significantly on the cultivar. Internode explants, but not leaf explants, of some cultivars, were able to produce adventitious shoots without treatment with growth regulator.
KW - in vitro cultivation
KW - Internode explant
KW - Leaf explant
KW - Plant age
KW - Shoot elongation
UR - http://www.scopus.com/inward/record.url?scp=33745726386&partnerID=8YFLogxK
U2 - 10.1007/s11240-005-9050-6
DO - 10.1007/s11240-005-9050-6
M3 - Article
AN - SCOPUS:33745726386
VL - 85
SP - 75
EP - 82
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -