Details
| Original language | English |
|---|---|
| Pages (from-to) | 823-832 |
| Number of pages | 10 |
| Journal | New Phytologist |
| Volume | 204 |
| Issue number | 4 |
| Publication status | Published - 26 Aug 2014 |
| Externally published | Yes |
Abstract
Summary: Transcription activator-like effectors (TALEs) from plant pathogenic Xanthomonas spp. and the related RipTALs from Ralstonia solanacearum are DNA-binding proteins with a modular DNA-binding domain. This domain is both predictable and programmable, which simplifies elucidation of TALE function in planta and facilitates generation of DNA-binding modules with desired specificity for biotechnological approaches. Recently identified TALE host target genes that either promote or stop bacterial disease provide new insights into how expression of TALE genes affects the plant-pathogen interaction. Since its elucidation the TALE code has been continuously refined and now provides a mature tool that, in combination with transcriptome profiling, allows rapid isolation of novel TALE target genes. The TALE code is also the basis for synthetic promoter-traps that mediate recognition of TALE or RipTAL proteins in engineered plants. In this review, we will summarize recent findings in plant-focused TALE research. In addition, we will provide an outline of the newly established gene isolation approach for TALE or RipTAL host target genes with an emphasis on potential pitfalls.
Keywords
- Effector, Genome editing, Plant pathogen, Resistance, Susceptibility, Transcription activator-like effector (TALE), Virulence
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: New Phytologist, Vol. 204, No. 4, 26.08.2014, p. 823-832.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - TAL effectors - pathogen strategies and plant resistance engineering
AU - Boch, Jens
AU - Bonas, Ulla
AU - Lahaye, Thomas
N1 - Publisher Copyright: © 2014 New Phytologist Trust. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2014/8/26
Y1 - 2014/8/26
N2 - Summary: Transcription activator-like effectors (TALEs) from plant pathogenic Xanthomonas spp. and the related RipTALs from Ralstonia solanacearum are DNA-binding proteins with a modular DNA-binding domain. This domain is both predictable and programmable, which simplifies elucidation of TALE function in planta and facilitates generation of DNA-binding modules with desired specificity for biotechnological approaches. Recently identified TALE host target genes that either promote or stop bacterial disease provide new insights into how expression of TALE genes affects the plant-pathogen interaction. Since its elucidation the TALE code has been continuously refined and now provides a mature tool that, in combination with transcriptome profiling, allows rapid isolation of novel TALE target genes. The TALE code is also the basis for synthetic promoter-traps that mediate recognition of TALE or RipTAL proteins in engineered plants. In this review, we will summarize recent findings in plant-focused TALE research. In addition, we will provide an outline of the newly established gene isolation approach for TALE or RipTAL host target genes with an emphasis on potential pitfalls.
AB - Summary: Transcription activator-like effectors (TALEs) from plant pathogenic Xanthomonas spp. and the related RipTALs from Ralstonia solanacearum are DNA-binding proteins with a modular DNA-binding domain. This domain is both predictable and programmable, which simplifies elucidation of TALE function in planta and facilitates generation of DNA-binding modules with desired specificity for biotechnological approaches. Recently identified TALE host target genes that either promote or stop bacterial disease provide new insights into how expression of TALE genes affects the plant-pathogen interaction. Since its elucidation the TALE code has been continuously refined and now provides a mature tool that, in combination with transcriptome profiling, allows rapid isolation of novel TALE target genes. The TALE code is also the basis for synthetic promoter-traps that mediate recognition of TALE or RipTAL proteins in engineered plants. In this review, we will summarize recent findings in plant-focused TALE research. In addition, we will provide an outline of the newly established gene isolation approach for TALE or RipTAL host target genes with an emphasis on potential pitfalls.
KW - Effector
KW - Genome editing
KW - Plant pathogen
KW - Resistance
KW - Susceptibility
KW - Transcription activator-like effector (TALE)
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=84911954031&partnerID=8YFLogxK
U2 - 10.1111/nph.13015
DO - 10.1111/nph.13015
M3 - Review article
C2 - 25539004
AN - SCOPUS:84911954031
VL - 204
SP - 823
EP - 832
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -