Details
| Original language | English |
|---|---|
| Pages (from-to) | 428-432 |
| Number of pages | 5 |
| Journal | Virulence |
| Volume | 1 |
| Issue number | 5 |
| Publication status | Published - 1 Sept 2010 |
| Externally published | Yes |
Abstract
TAL effectors are important virulence factors of bacterial plant pathogenic Xanthomonas, which infect a wide variety of plants including valuable crops like pepper, rice and citrus. TAL proteins are translocated via the bacterial type III secretion system into host cells and induce transcription of plant genes by binding to target gene promoters. Members of the TAL effector family differ mainly in their central domain of tandemly arranged repeats of typically 34 amino acids each with hypervariable di-amino acids at positions 12 and 13. We recently showed that target DNArecognition specificity of TAL effectors is encoded in a modular and clearly predictable mode. The repeats of TAL effectors feature a surprising one-repeat-to-one-bp correlation with different repeat types exhibiting a different DNA base pair specificity. Accordingly, we predicted DNA specificities of TAL effectors and generated artificial TAL proteins with novel DNA recognition specificities. We describe here novel artificial TALs and discuss implications for the DNA recognition specificity. The unique TAL-DNA binding domain allows design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.
Keywords
- Avrbs3, Biotechnology, Plant pathogen, Repeats, RVD, Transcriptional activator, Type III secretion, Xanthomonas
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Parasitology
- Immunology and Microbiology(all)
- Microbiology
- Immunology and Microbiology(all)
- Immunology
- Medicine(all)
- Microbiology (medical)
- Medicine(all)
- Infectious Diseases
Sustainable Development Goals
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In: Virulence, Vol. 1, No. 5, 01.09.2010, p. 428-432.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - TAL effector-DNA specificity
AU - Scholze, Heidi
AU - Boch, Jens
N1 - Funding Information: We thank A. Richter for constructs, A. Landgraf for excellent technical assistance and U. Bonas for continuous support. This work was supported by the Deutsche Forschungsgemeinschaft (SPP 1212). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - TAL effectors are important virulence factors of bacterial plant pathogenic Xanthomonas, which infect a wide variety of plants including valuable crops like pepper, rice and citrus. TAL proteins are translocated via the bacterial type III secretion system into host cells and induce transcription of plant genes by binding to target gene promoters. Members of the TAL effector family differ mainly in their central domain of tandemly arranged repeats of typically 34 amino acids each with hypervariable di-amino acids at positions 12 and 13. We recently showed that target DNArecognition specificity of TAL effectors is encoded in a modular and clearly predictable mode. The repeats of TAL effectors feature a surprising one-repeat-to-one-bp correlation with different repeat types exhibiting a different DNA base pair specificity. Accordingly, we predicted DNA specificities of TAL effectors and generated artificial TAL proteins with novel DNA recognition specificities. We describe here novel artificial TALs and discuss implications for the DNA recognition specificity. The unique TAL-DNA binding domain allows design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.
AB - TAL effectors are important virulence factors of bacterial plant pathogenic Xanthomonas, which infect a wide variety of plants including valuable crops like pepper, rice and citrus. TAL proteins are translocated via the bacterial type III secretion system into host cells and induce transcription of plant genes by binding to target gene promoters. Members of the TAL effector family differ mainly in their central domain of tandemly arranged repeats of typically 34 amino acids each with hypervariable di-amino acids at positions 12 and 13. We recently showed that target DNArecognition specificity of TAL effectors is encoded in a modular and clearly predictable mode. The repeats of TAL effectors feature a surprising one-repeat-to-one-bp correlation with different repeat types exhibiting a different DNA base pair specificity. Accordingly, we predicted DNA specificities of TAL effectors and generated artificial TAL proteins with novel DNA recognition specificities. We describe here novel artificial TALs and discuss implications for the DNA recognition specificity. The unique TAL-DNA binding domain allows design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.
KW - Avrbs3
KW - Biotechnology
KW - Plant pathogen
KW - Repeats
KW - RVD
KW - Transcriptional activator
KW - Type III secretion
KW - Xanthomonas
UR - http://www.scopus.com/inward/record.url?scp=78349278103&partnerID=8YFLogxK
U2 - 10.4161/viru.1.5.12863
DO - 10.4161/viru.1.5.12863
M3 - Article
AN - SCOPUS:78349278103
VL - 1
SP - 428
EP - 432
JO - Virulence
JF - Virulence
SN - 2150-5594
IS - 5
ER -