Details
| Original language | English |
|---|---|
| Title of host publication | TALENs |
| Subtitle of host publication | Methods and Protocols |
| Pages | 9-25 |
| Number of pages | 17 |
| Publication status | Published - 2016 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1338 |
| ISSN (Print) | 1064-3745 |
Abstract
Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use
Keywords
- CRISPR, Genome editing, Genome engineering, TALE, TALEN, Xanthomonas
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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TALENs: Methods and Protocols. 2016. p. 9-25 (Methods in Molecular Biology; Vol. 1338).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - TAL effector DNA-binding principles and specificity
AU - Richter, Annekatrin
AU - Streubel, Jana
AU - Boch, Jens
N1 - Funding information: We thank U. Bonas and T. Schreiber for discussion and comments on the manuscript. This work was supported by DFG grants BO 1496/6-1 and BO1496/7-1 to JB.
PY - 2016
Y1 - 2016
N2 - Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use
AB - Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use
KW - CRISPR
KW - Genome editing
KW - Genome engineering
KW - TALE
KW - TALEN
KW - Xanthomonas
UR - http://www.scopus.com/inward/record.url?scp=84943568261&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2932-0_2
DO - 10.1007/978-1-4939-2932-0_2
M3 - Contribution to book/anthology
C2 - 26443210
AN - SCOPUS:84943568261
T3 - Methods in Molecular Biology
SP - 9
EP - 25
BT - TALENs
ER -