Details
| Original language | English |
|---|---|
| Pages (from-to) | 1272-1286 |
| Number of pages | 15 |
| Journal | Journal of applied microbiology |
| Volume | 129 |
| Issue number | 5 |
| Early online date | 13 May 2020 |
| Publication status | Published - 13 Oct 2020 |
Abstract
Aims: Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic peptides to assess their applicability for this purpose. Methods and Results: Two synthetic anti-endotoxin peptides, Pep19-2.5 and Pep19-4LF (Aspidasept I and II) were tested against both Gram-positive (Staphylococcus aureus and Streptococcus oralis) and Gram-negative (Pseudomonas aeruginosa and Aggregatibacter actinomycetemcomitans) bacteria associated with implant infections. Their activity was evaluated against different states of biofilm formation on the implant material titanium using CFU, live/dead fluorescence staining and confocal microscopy. Both peptides inhibited planktonic bacteria growth, impacted initial bacterial adhesion, reduced biofilm volume and increased the proportion of dead cells. Additionally, cytotoxicity analyses showed that neither peptide harmed human gingival fibroblasts nor osteoblasts at lower concentrations. Conclusion: A concentration-dependent antibacterial activity of both peptides against biofilms of four clinically relevant bacteria could be demonstrated. Significance and Impact of the Study: The results of this study serve as a promising basis for the improvement of these peptides in order to finally achieve a peptide-equipped antibacterial implant surface.
Keywords
- antimicrobials, biofilms, infection, microbial contamination, peptides
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of applied microbiology, Vol. 129, No. 5, 13.10.2020, p. 1272-1286.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Synthetic anti-endotoxin peptides interfere with Gram-positive and Gram-negative bacteria, their adhesion and biofilm formation on titanium
AU - Subh, L.
AU - Correa, W.
AU - Pinkvos, T. J.
AU - Behrens, P.
AU - Brandenburg, K.
AU - Gutsmann, T.
AU - Stiesch, M.
AU - Doll, K.
AU - Winkel, A.
N1 - Funding Information: This study was part of the Central Innovation Program for SMEs (ZIM-Zentrales Innovationsprogramm Mittelstand) project and was financially supported by the Federal Ministry of Economic Affairs and Energy, Germany. K.D. was supported by the research initiative Biofabrication for NIFE, funded by the State of Lower Saxony and the Volkswagen Foundation. W.C. was temporarily supported by a grant of Brandenburg Antiinfektiva GmbH. The authors thank Henning Hartwig for excellent technical assistance and the AristoTech Industries GmbH for their helpful suggestions towards this work.
PY - 2020/10/13
Y1 - 2020/10/13
N2 - Aims: Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic peptides to assess their applicability for this purpose. Methods and Results: Two synthetic anti-endotoxin peptides, Pep19-2.5 and Pep19-4LF (Aspidasept I and II) were tested against both Gram-positive (Staphylococcus aureus and Streptococcus oralis) and Gram-negative (Pseudomonas aeruginosa and Aggregatibacter actinomycetemcomitans) bacteria associated with implant infections. Their activity was evaluated against different states of biofilm formation on the implant material titanium using CFU, live/dead fluorescence staining and confocal microscopy. Both peptides inhibited planktonic bacteria growth, impacted initial bacterial adhesion, reduced biofilm volume and increased the proportion of dead cells. Additionally, cytotoxicity analyses showed that neither peptide harmed human gingival fibroblasts nor osteoblasts at lower concentrations. Conclusion: A concentration-dependent antibacterial activity of both peptides against biofilms of four clinically relevant bacteria could be demonstrated. Significance and Impact of the Study: The results of this study serve as a promising basis for the improvement of these peptides in order to finally achieve a peptide-equipped antibacterial implant surface.
AB - Aims: Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic peptides to assess their applicability for this purpose. Methods and Results: Two synthetic anti-endotoxin peptides, Pep19-2.5 and Pep19-4LF (Aspidasept I and II) were tested against both Gram-positive (Staphylococcus aureus and Streptococcus oralis) and Gram-negative (Pseudomonas aeruginosa and Aggregatibacter actinomycetemcomitans) bacteria associated with implant infections. Their activity was evaluated against different states of biofilm formation on the implant material titanium using CFU, live/dead fluorescence staining and confocal microscopy. Both peptides inhibited planktonic bacteria growth, impacted initial bacterial adhesion, reduced biofilm volume and increased the proportion of dead cells. Additionally, cytotoxicity analyses showed that neither peptide harmed human gingival fibroblasts nor osteoblasts at lower concentrations. Conclusion: A concentration-dependent antibacterial activity of both peptides against biofilms of four clinically relevant bacteria could be demonstrated. Significance and Impact of the Study: The results of this study serve as a promising basis for the improvement of these peptides in order to finally achieve a peptide-equipped antibacterial implant surface.
KW - antimicrobials
KW - biofilms
KW - infection
KW - microbial contamination
KW - peptides
UR - http://www.scopus.com/inward/record.url?scp=85087306410&partnerID=8YFLogxK
U2 - 10.1111/jam.14701
DO - 10.1111/jam.14701
M3 - Article
C2 - 32403180
AN - SCOPUS:85087306410
VL - 129
SP - 1272
EP - 1286
JO - Journal of applied microbiology
JF - Journal of applied microbiology
SN - 1364-5072
IS - 5
ER -