Details
| Original language | English |
|---|---|
| Pages (from-to) | 2353-2359 |
| Number of pages | 7 |
| Journal | BIOMACROMOLECULES |
| Volume | 9 |
| Issue number | 9 |
| Publication status | Published - 9 Aug 2008 |
Abstract
Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Biomaterials
- Materials Science(all)
- Polymers and Plastics
- Materials Science(all)
- Materials Chemistry
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In: BIOMACROMOLECULES, Vol. 9, No. 9, 09.08.2008, p. 2353-2359.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Synthesis and Biological Evaluation of a Polysialic Acid-Based Hydrogel as Enzymatically Degradable Scaffold Material for Tissue Engineering
AU - Berski, Silke
AU - van Bergeijk, Jeroen
AU - Schwarzer, David
AU - Stark, Yvonne
AU - Kasper, Cornelia
AU - Scheper, Thomas
AU - Grothe, Claudia
AU - Gerardy-Schahn, Rita
AU - Kirschning, Andreas
AU - Dräger, Gerald
PY - 2008/8/9
Y1 - 2008/8/9
N2 - Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
AB - Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
UR - http://www.scopus.com/inward/record.url?scp=52649149159&partnerID=8YFLogxK
U2 - 10.1021/bm800327s
DO - 10.1021/bm800327s
M3 - Article
C2 - 18690740
AN - SCOPUS:52649149159
VL - 9
SP - 2353
EP - 2359
JO - BIOMACROMOLECULES
JF - BIOMACROMOLECULES
SN - 1525-7797
IS - 9
ER -