Details
| Original language | English |
|---|---|
| Article number | lqae110 |
| Journal | NAR Genomics and Bioinformatics |
| Volume | 6 |
| Issue number | 3 |
| Early online date | 24 Aug 2024 |
| Publication status | Published - Sept 2024 |
Abstract
Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.
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In: NAR Genomics and Bioinformatics, Vol. 6, No. 3, lqae110, 09.2024.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Synonymous rpsH variants
T2 - the common denominator in Escherichia coli adapting to ionizing radiation
AU - Stemwedel, Katharina
AU - Haase, Nadin
AU - Christ, Simon
AU - Bogdanova, Natalia V
AU - Rudorf, Sophia
N1 - Publisher Copyright: © 2024 The Author(s). Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.
PY - 2024/9
Y1 - 2024/9
N2 - Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.
AB - Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.
UR - http://www.scopus.com/inward/record.url?scp=85202454533&partnerID=8YFLogxK
U2 - 10.1093/nargab/lqae110
DO - 10.1093/nargab/lqae110
M3 - Article
VL - 6
JO - NAR Genomics and Bioinformatics
JF - NAR Genomics and Bioinformatics
IS - 3
M1 - lqae110
ER -