TY - JOUR

T1 - Sustainable use of mangroves as sources of valuable medicinal compounds: Species identification, propagation and secondary metabolite composition

AU - Glasenapp, Y.

AU - Korth, I.

AU - Nguyen, X. V.

AU - Papenbrock, J.

N1 - Funding Information: We would like to thank Yvonne Leye and Fabian Söffker for their expertise in taking care for the mangrove plants. Many thanks to Niranjan Divakaran for contributing some mangrove ITS sequences during his stay in our laboratory. We would like to acknowledge Sofia Isabell Rupp-Schröder, Hannover, who performed the elemental analysis. We would like to thank researchers from following countries who contributed dried leaf material: Md. Amanat Ullah, Center for Environmental and Geographic Information Services (CEGIS), Bangladesh; Cesar Zacarías, Consejo Nacional de Áreas Protegidas (CONAP), Guatemala; Dr. Sayed Abu Bakr, director of natural protectorates, Western desert, Egypt; Prof. Ahmad K Hegazy, Prof. of Applied Ecology, Botany and Microbiology Department, Faculty of Science, Cairo University, Egypt; Centro de Investigaciones de Ecosistemas Costeros (CIEC) in Cayo Coco, Ciego de Avila, Cuba.

PY - 2019/3

Y1 - 2019/3

N2 - Mangroves are able to withstand a number of stress factors, such as high salt concentrations, tidal flooding, strong wind, solar radiation and heat. Their ability to grow under these circumstances is based on morphological and physiological adaptations, among them the high abundance of plant secondary metabolites. We are interested to investigate and exploit their medicinal and biotechnological potential for new bioactive compounds, without collecting material in the countries of origin and in a sustainable way. Therefore, a simple identification system based on molecular marker analysis, and a sustainable greenhouse propagation protocol for the continuous supply of fresh plant material, were established. DNA barcoding of the internal transcribed spacer (ITS) including ITS1, the 5.8S rRNA region and ITS2 as a molecular marker was applied for several mangrove species. The obtained data and GenBank sequences were used for species identification. Three mangrove species are cultivated in our greenhouse and propagated in different ways: Avicennia species produced many propagules in the greenhouse, however, further propagation by cuttings was not successful. Laguncularia racemosa was propagated by cuttings in a fog house whereas Bruguiera cylindrica was difficult to cultivate and propagation was not successful. Finally, the concentration of secondary phenolic compounds, including flavonoids, and the content of major elements were compared among naturally and greenhouse-grown mangroves indicating comparable amounts and composition.

AB - Mangroves are able to withstand a number of stress factors, such as high salt concentrations, tidal flooding, strong wind, solar radiation and heat. Their ability to grow under these circumstances is based on morphological and physiological adaptations, among them the high abundance of plant secondary metabolites. We are interested to investigate and exploit their medicinal and biotechnological potential for new bioactive compounds, without collecting material in the countries of origin and in a sustainable way. Therefore, a simple identification system based on molecular marker analysis, and a sustainable greenhouse propagation protocol for the continuous supply of fresh plant material, were established. DNA barcoding of the internal transcribed spacer (ITS) including ITS1, the 5.8S rRNA region and ITS2 as a molecular marker was applied for several mangrove species. The obtained data and GenBank sequences were used for species identification. Three mangrove species are cultivated in our greenhouse and propagated in different ways: Avicennia species produced many propagules in the greenhouse, however, further propagation by cuttings was not successful. Laguncularia racemosa was propagated by cuttings in a fog house whereas Bruguiera cylindrica was difficult to cultivate and propagation was not successful. Finally, the concentration of secondary phenolic compounds, including flavonoids, and the content of major elements were compared among naturally and greenhouse-grown mangroves indicating comparable amounts and composition.

KW - Avicennia

KW - DNA barcoding

KW - Fog house

KW - ITS marker

KW - Mangrove

KW - Propagation

KW - Secondary compounds

UR - http://www.scopus.com/inward/record.url?scp=85057616292&partnerID=8YFLogxK

U2 - 10.1016/j.sajb.2018.11.020

DO - 10.1016/j.sajb.2018.11.020

M3 - Article

AN - SCOPUS:85057616292

VL - 121

SP - 317

EP - 328

JO - South African journal of botany

JF - South African journal of botany

SN - 0254-6299

ER -