Details
| Original language | English |
|---|---|
| Pages (from-to) | 2305-2312 |
| Number of pages | 8 |
| Journal | Journal of experimental botany |
| Volume | 55 |
| Issue number | 406 |
| Publication status | Published - 1 Oct 2004 |
Abstract
Different field surveys have shown that sulphur (S) fertilization can increase the resistance of agricultural crops against fungal pathogens. The mechanisms of this sulphur-induced resistance (SIR) are, however, not yet known. Volatile S compounds are thought to play an important role because H 2S is toxic to fungi. A field experiment was conducted to analyse the influence of S fertilization and the activity of H2S-releasing enzymes on fungal infections. Two levels of N and S fertilizers and two varieties of oilseed rape were investigated with respect to their potential to release H2S by the enzymatic activity of L-cysteine desulphydrase (LCD) and O-acetyl-L-serine(thiol)lyase (OAS-TL). LCD releases H2S during cysteine degradation, while OAS-TL consumes H2S during cysteine synthesis and free H2S is only released in a side reaction. All plots of the field trial showed an infection with Pyrenopeziza brassicae and leaf disc samples were taken from visibly infected leaf areas and apparently uninfected areas to investigate the reaction to the infection in relation to the treatments. Different S fractions and the activities of LCD and OAS-TL were measured to evaluate the potential to release H2S in relation to S nutrition and fungal infection. S fertilization significantly increased the contents of total S, sulphate, organic S, cysteine, and glutathione in the plants, but decreased LCD activity. Infection with P. brassicae increased cysteine and glutathione contents, as well as the activity of LCD. Therefore crops were able to react to a fungal infection with a greater potential to release H2S, which is reflected by an increasing LCD activity with fungal infection.
Keywords
- Brassica napus L., HS, L-cysteine desulphydrase, O-acetyl-L-serine(thiol)lyase, Pyrenopeziza brassicae, Sulphur-induced resistance
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of experimental botany, Vol. 55, No. 406, 01.10.2004, p. 2305-2312.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Sulphur supply and infection with Pyrenopeziza brassicae influence L-cysteine desulphydrase activity in Brassica napus L.
AU - Bloem, Elke
AU - Riemenschneider, Anja
AU - Volker, Julia
AU - Papenbrock, Jutta
AU - Schmidt, Ahlert
AU - Salac, Ioana
AU - Haneklaus, Silvia
AU - Schnug, Ewald
N1 - Funding information: The work was financially supported by the German Research Foundation (DFG) in the sulphur research group 383, and by funds of the chemical industry, Germany.
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Different field surveys have shown that sulphur (S) fertilization can increase the resistance of agricultural crops against fungal pathogens. The mechanisms of this sulphur-induced resistance (SIR) are, however, not yet known. Volatile S compounds are thought to play an important role because H 2S is toxic to fungi. A field experiment was conducted to analyse the influence of S fertilization and the activity of H2S-releasing enzymes on fungal infections. Two levels of N and S fertilizers and two varieties of oilseed rape were investigated with respect to their potential to release H2S by the enzymatic activity of L-cysteine desulphydrase (LCD) and O-acetyl-L-serine(thiol)lyase (OAS-TL). LCD releases H2S during cysteine degradation, while OAS-TL consumes H2S during cysteine synthesis and free H2S is only released in a side reaction. All plots of the field trial showed an infection with Pyrenopeziza brassicae and leaf disc samples were taken from visibly infected leaf areas and apparently uninfected areas to investigate the reaction to the infection in relation to the treatments. Different S fractions and the activities of LCD and OAS-TL were measured to evaluate the potential to release H2S in relation to S nutrition and fungal infection. S fertilization significantly increased the contents of total S, sulphate, organic S, cysteine, and glutathione in the plants, but decreased LCD activity. Infection with P. brassicae increased cysteine and glutathione contents, as well as the activity of LCD. Therefore crops were able to react to a fungal infection with a greater potential to release H2S, which is reflected by an increasing LCD activity with fungal infection.
AB - Different field surveys have shown that sulphur (S) fertilization can increase the resistance of agricultural crops against fungal pathogens. The mechanisms of this sulphur-induced resistance (SIR) are, however, not yet known. Volatile S compounds are thought to play an important role because H 2S is toxic to fungi. A field experiment was conducted to analyse the influence of S fertilization and the activity of H2S-releasing enzymes on fungal infections. Two levels of N and S fertilizers and two varieties of oilseed rape were investigated with respect to their potential to release H2S by the enzymatic activity of L-cysteine desulphydrase (LCD) and O-acetyl-L-serine(thiol)lyase (OAS-TL). LCD releases H2S during cysteine degradation, while OAS-TL consumes H2S during cysteine synthesis and free H2S is only released in a side reaction. All plots of the field trial showed an infection with Pyrenopeziza brassicae and leaf disc samples were taken from visibly infected leaf areas and apparently uninfected areas to investigate the reaction to the infection in relation to the treatments. Different S fractions and the activities of LCD and OAS-TL were measured to evaluate the potential to release H2S in relation to S nutrition and fungal infection. S fertilization significantly increased the contents of total S, sulphate, organic S, cysteine, and glutathione in the plants, but decreased LCD activity. Infection with P. brassicae increased cysteine and glutathione contents, as well as the activity of LCD. Therefore crops were able to react to a fungal infection with a greater potential to release H2S, which is reflected by an increasing LCD activity with fungal infection.
KW - Brassica napus L.
KW - HS
KW - L-cysteine desulphydrase
KW - O-acetyl-L-serine(thiol)lyase
KW - Pyrenopeziza brassicae
KW - Sulphur-induced resistance
UR - http://www.scopus.com/inward/record.url?scp=5144222717&partnerID=8YFLogxK
U2 - 10.1093/jxb/erh236
DO - 10.1093/jxb/erh236
M3 - Article
C2 - 15310816
AN - SCOPUS:5144222717
VL - 55
SP - 2305
EP - 2312
JO - Journal of experimental botany
JF - Journal of experimental botany
SN - 0022-0957
IS - 406
ER -