Details
| Original language | English |
|---|---|
| Pages (from-to) | 977-986 |
| Number of pages | 10 |
| Journal | Journal of Tissue Engineering and Regenerative Medicine |
| Volume | 16 |
| Issue number | 11 |
| Early online date | 13 Aug 2022 |
| Publication status | Published - Nov 2022 |
Abstract
Three-dimensional (3D) cultivation platforms allow the creation of cell models, which more closely resemble in vivo-like cell behavior. Therefore, 3D cell culture platforms have started to replace conventional two-dimensional (2D) cultivation techniques in many fields. Besides the advantages of 3D culture, there are also some challenges: cultivation in 3D often results in an inhomogeneous microenvironment and therefore unique cultivation conditions for each cell inside the construct. As a result, the analysis and precise control over the singular cell state is limited in 3D. In this work, we address these challenges by exploring ways to monitor oxygen concentrations in gelatin methacryloyl (GelMA) 3D hydrogel culture at the cellular level using hypoxia reporter cells and deep within the construct using a non-invasive optical oxygen sensing spot. We could show that the appearance of oxygen limitations is more prominent in softer GelMA-hydrogels, which enable better cell spreading. Beyond demonstrating novel or space-resolved techniques of visualizing oxygen availability in hydrogel constructs, we also describe a method to create a stable and controlled oxygen gradient throughout the construct using a 3D printed flow-through chamber.
Keywords
- 3D cell culture, AD-MSCs, hydrogels, hypoxia reporter cells, hypoxia sensor, oxygen concentration measurements
ASJC Scopus subject areas
- Medicine(all)
- Medicine (miscellaneous)
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 16, No. 11, 11.2022, p. 977-986.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Studies on oxygen availability and the creation of natural and artificial oxygen gradients in gelatin-methacryloyl hydrogel 3D cell culture
AU - Schmitz, Carola
AU - Pepelanova, Iliyana
AU - Ude, Christian
AU - Lavrentieva, Antonina
N1 - Funding Information: This study was funded by the German Research Foundation (DFG Project 398007461 488 “3D Dual‐Gradient Systems for Functional Cell Screening” and “BiomolecularSensor Platform for Elucidating Hypoxic Signatures in 2D and 3D in vitro culture Systems”). This work was also supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany through the SMART BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover. Moreover, we would like to thank Dr. Janina Bahnemann and Steffen Winkler, who provided the 3D printing setup as well as Vsevolod V. Belousov and Ekaterina Potekhina for providing sensor plasmids.
PY - 2022/11
Y1 - 2022/11
N2 - Three-dimensional (3D) cultivation platforms allow the creation of cell models, which more closely resemble in vivo-like cell behavior. Therefore, 3D cell culture platforms have started to replace conventional two-dimensional (2D) cultivation techniques in many fields. Besides the advantages of 3D culture, there are also some challenges: cultivation in 3D often results in an inhomogeneous microenvironment and therefore unique cultivation conditions for each cell inside the construct. As a result, the analysis and precise control over the singular cell state is limited in 3D. In this work, we address these challenges by exploring ways to monitor oxygen concentrations in gelatin methacryloyl (GelMA) 3D hydrogel culture at the cellular level using hypoxia reporter cells and deep within the construct using a non-invasive optical oxygen sensing spot. We could show that the appearance of oxygen limitations is more prominent in softer GelMA-hydrogels, which enable better cell spreading. Beyond demonstrating novel or space-resolved techniques of visualizing oxygen availability in hydrogel constructs, we also describe a method to create a stable and controlled oxygen gradient throughout the construct using a 3D printed flow-through chamber.
AB - Three-dimensional (3D) cultivation platforms allow the creation of cell models, which more closely resemble in vivo-like cell behavior. Therefore, 3D cell culture platforms have started to replace conventional two-dimensional (2D) cultivation techniques in many fields. Besides the advantages of 3D culture, there are also some challenges: cultivation in 3D often results in an inhomogeneous microenvironment and therefore unique cultivation conditions for each cell inside the construct. As a result, the analysis and precise control over the singular cell state is limited in 3D. In this work, we address these challenges by exploring ways to monitor oxygen concentrations in gelatin methacryloyl (GelMA) 3D hydrogel culture at the cellular level using hypoxia reporter cells and deep within the construct using a non-invasive optical oxygen sensing spot. We could show that the appearance of oxygen limitations is more prominent in softer GelMA-hydrogels, which enable better cell spreading. Beyond demonstrating novel or space-resolved techniques of visualizing oxygen availability in hydrogel constructs, we also describe a method to create a stable and controlled oxygen gradient throughout the construct using a 3D printed flow-through chamber.
KW - 3D cell culture
KW - AD-MSCs
KW - hydrogels
KW - hypoxia reporter cells
KW - hypoxia sensor
KW - oxygen concentration measurements
UR - http://www.scopus.com/inward/record.url?scp=85135804241&partnerID=8YFLogxK
U2 - 10.1002/term.3344
DO - 10.1002/term.3344
M3 - Article
C2 - 35962761
AN - SCOPUS:85135804241
VL - 16
SP - 977
EP - 986
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 11
ER -