Details
| Original language | English |
|---|---|
| Pages (from-to) | 180-184 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 53 |
| Issue number | 2 |
| Publication status | Published - Feb 2000 |
Abstract
The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen catabolite repression of enzyme formation was absent in this bacterium. Cultivation in a reactor showed that the dissolved oxygen level is the limiting factor for L-asparaginase production by E. aerogenes. Glucose was found to be a repressor of enzyme synthesis. Asparagine was absent intracellularly when the L-asparaginase level was high. An increase in the extracellular alanine level when the dissolved oxygen remained low indicated a shift from aerobic to fermentative metabolism.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 53, No. 2, 02.2000, p. 180-184.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Studies on nutritional and oxygen requirements for production of L- asparaginase by Enterobacter aerogenes
AU - Mukherjee, J.
AU - Majumdar, S.
AU - Scheper, T.
N1 - Funding information: The first author is thankful to the Deutscher Akademischer Austauschdienst, Germany and the Council of Scientific and Industrial Research, India for their financial support.
PY - 2000/2
Y1 - 2000/2
N2 - The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen catabolite repression of enzyme formation was absent in this bacterium. Cultivation in a reactor showed that the dissolved oxygen level is the limiting factor for L-asparaginase production by E. aerogenes. Glucose was found to be a repressor of enzyme synthesis. Asparagine was absent intracellularly when the L-asparaginase level was high. An increase in the extracellular alanine level when the dissolved oxygen remained low indicated a shift from aerobic to fermentative metabolism.
AB - The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen catabolite repression of enzyme formation was absent in this bacterium. Cultivation in a reactor showed that the dissolved oxygen level is the limiting factor for L-asparaginase production by E. aerogenes. Glucose was found to be a repressor of enzyme synthesis. Asparagine was absent intracellularly when the L-asparaginase level was high. An increase in the extracellular alanine level when the dissolved oxygen remained low indicated a shift from aerobic to fermentative metabolism.
UR - http://www.scopus.com/inward/record.url?scp=0034005352&partnerID=8YFLogxK
U2 - 10.1007/s002530050006
DO - 10.1007/s002530050006
M3 - Article
C2 - 10709980
AN - SCOPUS:0034005352
VL - 53
SP - 180
EP - 184
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -