Details
| Original language | English |
|---|---|
| Pages (from-to) | 1043-1052 |
| Number of pages | 10 |
| Journal | Journal of Biophotonics |
| Volume | 10 |
| Issue number | 8 |
| Publication status | Published - 11 Aug 2017 |
Abstract
There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser-based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants. (Figure presented.).
Keywords
- biofilm, gold, laser ablation, pulsed lasers
ASJC Scopus subject areas
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In: Journal of Biophotonics, Vol. 10, No. 8, 11.08.2017, p. 1043-1052.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Structural damage of Bacillus subtilis biofilms using pulsed laser interaction with gold thin films
AU - Krawinkel, Judith
AU - Torres, Maria Leilani
AU - Mhatre, Eisha
AU - Kovács, Ákos T.
AU - Heisterkamp, Alexander
N1 - Funding Information: We acknowledge Tetyana Melnyk for helping us in the SEM imaging. We thank Tobias Birr for the helpful discussions on calculations of gold film thickness. We are grateful to Coherent GmbH for lending us the Helios laser. We also thank Prof. Kürsad Turgay and Heinrich Schäffer for providing additional samples. Work in the laboratory of A. T. K. is supported by Grants KO4741/2-1 and KO4741/3-1 from the Deutsche Forschungsgemeinschaft (DFG). E. M. was supported by Jena School for Microbial Communications (JSMC). The LSM780 microscope was financed by a grant from Thüringer Ministerium für Bildung, Wissenschaft und Kultur (project B11024-715). A.H acknowledges the financial support of Excellence Cluster, REBIRTH and the funding initiative “Biofabrication for NIFE”.
PY - 2017/8/11
Y1 - 2017/8/11
N2 - There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser-based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants. (Figure presented.).
AB - There is a huge interest in developing strategies to effectively eliminate biofilms due to their negative impact in both industrial and clinical settings. In this study, structural damage was induced on two day-old B. subtilis biofilms using the interaction of 532 nm pulsed laser with gold thin films. Radiant exposure of 225 mJ/cm2 induced distinct changes on the surface structure and overall morphology of the matured biofilms after laser irradiation. Moreover, at the radiant exposure used, changes in the colour and viscosity of the biofilm were observed which may indicate a compromised extracellular matrix. Irradiated biofilms in the presence of gold film also showed strong propidium iodide signal which implies an increase in the number of dead bacterial cells after laser treatment. Thus, this laser-based technique is a promising approach in targeting and eradicating matured biofilms attached on surfaces such as medical implants. (Figure presented.).
KW - biofilm
KW - gold
KW - laser ablation
KW - pulsed lasers
UR - http://www.scopus.com/inward/record.url?scp=84990934149&partnerID=8YFLogxK
U2 - 10.1002/jbio.201600146
DO - 10.1002/jbio.201600146
M3 - Article
C2 - 27714933
AN - SCOPUS:84990934149
VL - 10
SP - 1043
EP - 1052
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 8
ER -