Details
| Original language | English |
|---|---|
| Pages (from-to) | 383-386 |
| Number of pages | 4 |
| Journal | Nature Chemical Biology |
| Volume | 16 |
| Early online date | 17 Feb 2020 |
| Publication status | Published - Apr 2020 |
Abstract
Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Nature Chemical Biology, Vol. 16, 04.2020, p. 383-386.
Research output: Contribution to journal › Short/Brief/Rapid Communication › Research › peer review
}
TY - JOUR
T1 - Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids
AU - Caputi, Lorenzo
AU - Franke, Jakob
AU - Bussey, Kate
AU - Farrow, Scott C.
AU - Vieira, Ivo Jose Curcino
AU - Stevenson, Clare E. M.
AU - Lawson, David M.
AU - O’Connor, Sarah E.
N1 - Funding Information: S.E.O. acknowledges ERC (788301). J.F. acknowledges financial support by the SMART BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover, supported by the Ministry for Science and Culture (MWK) of Lower Saxony, Germany. We acknowledge Diamond Light Source for access to beamline I03 under proposal MX13467 with support from the European Community’s Seventh Framework Program (No. FP7/2007–2013) under Grant Agreement No. 283570 (BioStruct-X).
PY - 2020/4
Y1 - 2020/4
N2 - Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.
AB - Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.
UR - http://www.scopus.com/inward/record.url?scp=85079708576&partnerID=8YFLogxK
U2 - 10.1038/s41589-019-0460-x
DO - 10.1038/s41589-019-0460-x
M3 - Short/Brief/Rapid Communication
C2 - 32066966
AN - SCOPUS:85079708576
VL - 16
SP - 383
EP - 386
JO - Nature Chemical Biology
JF - Nature Chemical Biology
SN - 1552-4450
ER -