Details
| Original language | English |
|---|---|
| Pages (from-to) | 529-538 |
| Number of pages | 10 |
| Journal | MYCOLOGIA |
| Volume | 100 |
| Issue number | 4 |
| Publication status | Published - Jul 2008 |
Abstract
Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
Keywords
- 2-D electrophoresis, Basidiomycete, cDNA, De novo sequencing
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: MYCOLOGIA, Vol. 100, No. 4, 07.2008, p. 529-538.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Stress response of Nidula niveo-tomentosa to UV-A light
AU - Taupp, Daniela E.
AU - Nimtz, Manfred
AU - Berger, Ralf G.
AU - Zorn, Holger
PY - 2008/7
Y1 - 2008/7
N2 - Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
AB - Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveo-tomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
KW - 2-D electrophoresis
KW - Basidiomycete
KW - cDNA
KW - De novo sequencing
UR - http://www.scopus.com/inward/record.url?scp=54049118783&partnerID=8YFLogxK
U2 - 10.3852/07-179R
DO - 10.3852/07-179R
M3 - Article
C2 - 18833746
AN - SCOPUS:54049118783
VL - 100
SP - 529
EP - 538
JO - MYCOLOGIA
JF - MYCOLOGIA
SN - 0027-5514
IS - 4
ER -