Details
| Original language | English |
|---|---|
| Pages (from-to) | 73-92 |
| Number of pages | 20 |
| Journal | Advances in Biochemical Engineering/Biotechnology |
| Volume | 89 |
| Publication status | Published - 20 Apr 2004 |
| Externally published | Yes |
Abstract
Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Advances in Biochemical Engineering/Biotechnology, Vol. 89, 20.04.2004, p. 73-92.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Stress induced by recombinant protein production in Escherichia coli.
AU - Hoffmann, Frank
AU - Rinas, Ursula
PY - 2004/4/20
Y1 - 2004/4/20
N2 - Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.
AB - Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.
UR - http://www.scopus.com/inward/record.url?scp=2942755215&partnerID=8YFLogxK
U2 - 10.1007/b93994
DO - 10.1007/b93994
M3 - Review article
C2 - 15217156
AN - SCOPUS:2942755215
VL - 89
SP - 73
EP - 92
JO - Advances in Biochemical Engineering/Biotechnology
JF - Advances in Biochemical Engineering/Biotechnology
SN - 0724-6145
ER -