Details
| Original language | English |
|---|---|
| Article number | e0199867 |
| Journal | PLOS ONE |
| Volume | 13 |
| Issue number | 7 |
| Publication status | Published - 5 Jul 2018 |
Abstract
Cryopreservation of biological materials is predominantly done using liquid nitrogen, and its application involves high maintenance costs and the need for periodical refilling of liquid nitrogen. Stable storage in mechanical freezers at −80C would eliminate these issues and allow for shipment of frozen specimens using dry ice. In this work, the possibility of increasing the storage temperature of cryopreserved samples to −80C by using combinations of DMSO and sucrose has been studied. Preservation efficacy was studied by measuring stability of liposomes encapsulated with carboxyfluorescein during storage at −150, −80 and −25C for up to three months. Thermal and molecular mobility properties of the different DMSO-sucrose formulations were measured using differential scanning calorimetry, whereas hydrogen bonding interactions of the formulations were probed by Fourier transform infrared spectroscopy. It was found that addition of sucrose to DMSO solutions increases the Tg, and decreases molecular mobility in the glassy state at a particular temperature. Although it was expected that storage above or close to Tg at −80C would affect liposome stability, stability was found to be similar compared to that of samples stored at −150C. Higher molecular mobility in the glassy state could not be associated with faster CF-leakage rates. Distinct differences in storage stability at −25C, far above Tg, were found among the sucrose/DMSO formulations, which were explained by the differences in permeability of sucrose and DMSO resulting in different levels of osmotic stress in the formulations.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
- General
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In: PLOS ONE, Vol. 13, No. 7, e0199867, 05.07.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Storage stability of liposomes stored at elevated subzero temperatures in DMSO/ sucrose mixtures
AU - Sydykov, Bulat
AU - Oldenhof, Harriëtte
AU - Sieme, Harald
AU - Wolkers, Willem F.
N1 - Funding: This work was financially supported by the German Research Foundation (DFG: Deutsche Forschungsgemeinschaft) via the Cluster of Excellence "From regenerative biology to reconstructive therapy" (REBIRTH, EXC 62/1) and grants WO1735/6-1 to WW and SI1462/4-1 to HS, and the publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2018/7/5
Y1 - 2018/7/5
N2 - Cryopreservation of biological materials is predominantly done using liquid nitrogen, and its application involves high maintenance costs and the need for periodical refilling of liquid nitrogen. Stable storage in mechanical freezers at −80C would eliminate these issues and allow for shipment of frozen specimens using dry ice. In this work, the possibility of increasing the storage temperature of cryopreserved samples to −80C by using combinations of DMSO and sucrose has been studied. Preservation efficacy was studied by measuring stability of liposomes encapsulated with carboxyfluorescein during storage at −150, −80 and −25C for up to three months. Thermal and molecular mobility properties of the different DMSO-sucrose formulations were measured using differential scanning calorimetry, whereas hydrogen bonding interactions of the formulations were probed by Fourier transform infrared spectroscopy. It was found that addition of sucrose to DMSO solutions increases the Tg, and decreases molecular mobility in the glassy state at a particular temperature. Although it was expected that storage above or close to Tg at −80C would affect liposome stability, stability was found to be similar compared to that of samples stored at −150C. Higher molecular mobility in the glassy state could not be associated with faster CF-leakage rates. Distinct differences in storage stability at −25C, far above Tg, were found among the sucrose/DMSO formulations, which were explained by the differences in permeability of sucrose and DMSO resulting in different levels of osmotic stress in the formulations.
AB - Cryopreservation of biological materials is predominantly done using liquid nitrogen, and its application involves high maintenance costs and the need for periodical refilling of liquid nitrogen. Stable storage in mechanical freezers at −80C would eliminate these issues and allow for shipment of frozen specimens using dry ice. In this work, the possibility of increasing the storage temperature of cryopreserved samples to −80C by using combinations of DMSO and sucrose has been studied. Preservation efficacy was studied by measuring stability of liposomes encapsulated with carboxyfluorescein during storage at −150, −80 and −25C for up to three months. Thermal and molecular mobility properties of the different DMSO-sucrose formulations were measured using differential scanning calorimetry, whereas hydrogen bonding interactions of the formulations were probed by Fourier transform infrared spectroscopy. It was found that addition of sucrose to DMSO solutions increases the Tg, and decreases molecular mobility in the glassy state at a particular temperature. Although it was expected that storage above or close to Tg at −80C would affect liposome stability, stability was found to be similar compared to that of samples stored at −150C. Higher molecular mobility in the glassy state could not be associated with faster CF-leakage rates. Distinct differences in storage stability at −25C, far above Tg, were found among the sucrose/DMSO formulations, which were explained by the differences in permeability of sucrose and DMSO resulting in different levels of osmotic stress in the formulations.
UR - http://www.scopus.com/inward/record.url?scp=85049496562&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0199867
DO - 10.1371/journal.pone.0199867
M3 - Article
C2 - 29975741
AN - SCOPUS:85049496562
VL - 13
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 7
M1 - e0199867
ER -