Details
| Original language | English |
|---|---|
| Article number | 183 |
| Journal | Separations |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 8 Mar 2023 |
Abstract
Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SXC is related to PEG-mediated protein precipitation, but investigation of further process parameters was crucial to gain a better understanding of the SXC method. After explaining mechanistic fundamentals and their discovery, this review summarizes the findings on SXC from its first reference 11 years ago until today. Different applications of SXC are presented, demonstrating that the method can be used for a wide variety of targets and achieves high recovery rates and impurity removal. Further, critical process parameters for successful process implementation are discussed, including technical requirements, buffer composition, and scalability.
Keywords
- downstream processing, polyethylene glycol, purification, steric exclusion chromatography
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Chemical Engineering(all)
- Filtration and Separation
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In: Separations, Vol. 10, No. 3, 183, 08.03.2023.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Steric Exclusion Chromatography for Purification of Biomolecules
T2 - A Review
AU - Labisch, Jennifer J.
AU - Wiese, G. Philip
AU - Pflanz, Karl
N1 - Funding Information: This research was funded by Sartorius Stedim Biotech GmbH.
PY - 2023/3/8
Y1 - 2023/3/8
N2 - Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SXC is related to PEG-mediated protein precipitation, but investigation of further process parameters was crucial to gain a better understanding of the SXC method. After explaining mechanistic fundamentals and their discovery, this review summarizes the findings on SXC from its first reference 11 years ago until today. Different applications of SXC are presented, demonstrating that the method can be used for a wide variety of targets and achieves high recovery rates and impurity removal. Further, critical process parameters for successful process implementation are discussed, including technical requirements, buffer composition, and scalability.
AB - Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SXC is related to PEG-mediated protein precipitation, but investigation of further process parameters was crucial to gain a better understanding of the SXC method. After explaining mechanistic fundamentals and their discovery, this review summarizes the findings on SXC from its first reference 11 years ago until today. Different applications of SXC are presented, demonstrating that the method can be used for a wide variety of targets and achieves high recovery rates and impurity removal. Further, critical process parameters for successful process implementation are discussed, including technical requirements, buffer composition, and scalability.
KW - downstream processing
KW - polyethylene glycol
KW - purification
KW - steric exclusion chromatography
UR - http://www.scopus.com/inward/record.url?scp=85151164666&partnerID=8YFLogxK
U2 - 10.3390/separations10030183
DO - 10.3390/separations10030183
M3 - Review article
AN - SCOPUS:85151164666
VL - 10
JO - Separations
JF - Separations
IS - 3
M1 - 183
ER -