Details
| Original language | English |
|---|---|
| Pages (from-to) | 69-78 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 1176 |
| Issue number | 1-2 |
| Early online date | 1 Nov 2007 |
| Publication status | Published - 28 Dec 2007 |
| Externally published | Yes |
Abstract
The simulated moving bed (SMB) technology is a proven tool for efficient separation of binary mixtures. However, relying on isocratic conditions limits the applicability of the classical SMB approach when considering the field of bioseparations. Here, the use of gradients opens up new possibilities. A gradient in a SMB process can be established by using different solvent strengths in the incoming feed and desorbent streams, resulting in two internal plateaus of elution strength. Thus, compared to the conventional process, the overall amount of solvent needed can be reduced, productivity can be increased and more concentrated product streams can be obtained. In this contribution, two case studies will be presented. At first, the separation of bovine IgG from lysozyme will be analyzed as a model system. Antibodies are a common target substance in bio-chromatography, as therapeutic monoclonal antibodies are among the most promising biopharmaceuticals. Using adsorption data obtained from single-column experiments, an appropriate SMB process was designed and implemented. The second target component is the active dimeric form of the bone morphogenetic protein-2 (BMP-2). This protein was isolated from a renaturation solution, which also contained its inactive monomeric form as well as other undefined proteins from the bacterial production strain. A 3-zone open-loop gradient-SMB approach was used successfully for both separations.
Keywords
- Antibodies, BMP-2, Equilibration, Gradients, Moving-column implementation, Open-loop, Protein separation, Simulated moving bed
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
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In: Journal of Chromatography A, Vol. 1176, No. 1-2, 28.12.2007, p. 69-78.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Step gradients in 3-zone simulated moving bed chromatography. Application to the purification of antibodies and bone morphogenetic protein-2
AU - Keßler, Lars Christian
AU - Gueorguieva, Ludmila
AU - Rinas, Ursula
AU - Seidel-Morgenstern, Andreas
N1 - Funding Information: The support of Deutsche Forschungsgemeinschaft/SFB 578, Fonds der Chemischen Industrie and “Bayer-Schering AG” is gratefully acknowledged.
PY - 2007/12/28
Y1 - 2007/12/28
N2 - The simulated moving bed (SMB) technology is a proven tool for efficient separation of binary mixtures. However, relying on isocratic conditions limits the applicability of the classical SMB approach when considering the field of bioseparations. Here, the use of gradients opens up new possibilities. A gradient in a SMB process can be established by using different solvent strengths in the incoming feed and desorbent streams, resulting in two internal plateaus of elution strength. Thus, compared to the conventional process, the overall amount of solvent needed can be reduced, productivity can be increased and more concentrated product streams can be obtained. In this contribution, two case studies will be presented. At first, the separation of bovine IgG from lysozyme will be analyzed as a model system. Antibodies are a common target substance in bio-chromatography, as therapeutic monoclonal antibodies are among the most promising biopharmaceuticals. Using adsorption data obtained from single-column experiments, an appropriate SMB process was designed and implemented. The second target component is the active dimeric form of the bone morphogenetic protein-2 (BMP-2). This protein was isolated from a renaturation solution, which also contained its inactive monomeric form as well as other undefined proteins from the bacterial production strain. A 3-zone open-loop gradient-SMB approach was used successfully for both separations.
AB - The simulated moving bed (SMB) technology is a proven tool for efficient separation of binary mixtures. However, relying on isocratic conditions limits the applicability of the classical SMB approach when considering the field of bioseparations. Here, the use of gradients opens up new possibilities. A gradient in a SMB process can be established by using different solvent strengths in the incoming feed and desorbent streams, resulting in two internal plateaus of elution strength. Thus, compared to the conventional process, the overall amount of solvent needed can be reduced, productivity can be increased and more concentrated product streams can be obtained. In this contribution, two case studies will be presented. At first, the separation of bovine IgG from lysozyme will be analyzed as a model system. Antibodies are a common target substance in bio-chromatography, as therapeutic monoclonal antibodies are among the most promising biopharmaceuticals. Using adsorption data obtained from single-column experiments, an appropriate SMB process was designed and implemented. The second target component is the active dimeric form of the bone morphogenetic protein-2 (BMP-2). This protein was isolated from a renaturation solution, which also contained its inactive monomeric form as well as other undefined proteins from the bacterial production strain. A 3-zone open-loop gradient-SMB approach was used successfully for both separations.
KW - Antibodies
KW - BMP-2
KW - Equilibration
KW - Gradients
KW - Moving-column implementation
KW - Open-loop
KW - Protein separation
KW - Simulated moving bed
UR - http://www.scopus.com/inward/record.url?scp=36549007412&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2007.10.087
DO - 10.1016/j.chroma.2007.10.087
M3 - Article
C2 - 18036537
AN - SCOPUS:36549007412
VL - 1176
SP - 69
EP - 78
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -