Details
| Original language | English |
|---|---|
| Pages (from-to) | 62-74 |
| Number of pages | 13 |
| Journal | Biotechnology journal |
| Volume | 5 |
| Issue number | 1 |
| Publication status | Published - 12 Jan 2010 |
Abstract
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
Keywords
- Assay validation, Linear parallel-line assay, Mixed model approach, Non-linear parallel-line assay, Variance component analysis
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
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In: Biotechnology journal, Vol. 5, No. 1, 12.01.2010, p. 62-74.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays
AU - Zimmermann, Hanne
AU - Gerhard, Daniel
AU - Dingermann, Theo
AU - Hothorn, Ludwig A.
PY - 2010/1/12
Y1 - 2010/1/12
N2 - Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
AB - Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
KW - Assay validation
KW - Linear parallel-line assay
KW - Mixed model approach
KW - Non-linear parallel-line assay
KW - Variance component analysis
UR - http://www.scopus.com/inward/record.url?scp=75649098212&partnerID=8YFLogxK
U2 - 10.1002/biot.200900146
DO - 10.1002/biot.200900146
M3 - Article
C2 - 19844917
AN - SCOPUS:75649098212
VL - 5
SP - 62
EP - 74
JO - Biotechnology journal
JF - Biotechnology journal
SN - 1860-6768
IS - 1
ER -