Details
| Original language | English |
|---|---|
| Article number | 2889 |
| Number of pages | 11 |
| Journal | Sensors |
| Volume | 20 |
| Issue number | 10 |
| Publication status | Published - 20 May 2020 |
Abstract
We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.
Keywords
- Aptamer, Biosensor, Gold-nanoparticle, Lab-on-a-chip, Multi-mode, Planar-optical, Sensor, Surface plasmon resonance (SPR), Waveguide
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Physics and Astronomy(all)
- Instrumentation
- Engineering(all)
- Electrical and Electronic Engineering
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In: Sensors, Vol. 20, No. 10, 2889, 20.05.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement
AU - Walter, Johanna-Gabriela
AU - Eilers, Alina
AU - Alwis, Lourdes Shanika Malindi
AU - Roth, Bernhard Wilhelm
AU - Bremer, Kort
N1 - Funding information: B.W.R. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). J.-G.W. and K.B. acknowledge support from the Bundesministerium für Wirtschaft und Energie (BMWi) as well as the European Social Fund (ESF) within Grant Number 03EFHNI059. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. B.W.R. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany?s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). J.-G.W. and K.B. acknowledge support from the Bundesministerium f?r Wirtschaft und Energie (BMWi) as well as the European Social Fund (ESF) within Grant Number 03EFHNI059. The publication of this article was funded by the Open Access Fund of Leibniz Universit?t Hannover.
PY - 2020/5/20
Y1 - 2020/5/20
N2 - We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.
AB - We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.
KW - Aptamer
KW - Biosensor
KW - Gold-nanoparticle
KW - Lab-on-a-chip
KW - Multi-mode
KW - Planar-optical
KW - Sensor
KW - Surface plasmon resonance (SPR)
KW - Waveguide
UR - http://www.scopus.com/inward/record.url?scp=85085241832&partnerID=8YFLogxK
U2 - 10.3390/s20102889
DO - 10.3390/s20102889
M3 - Article
C2 - 32443702
AN - SCOPUS:85085241832
VL - 20
JO - Sensors
JF - Sensors
SN - 1424-8220
IS - 10
M1 - 2889
ER -