Details
| Original language | English |
|---|---|
| Pages (from-to) | 651-666 |
| Number of pages | 16 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 409 |
| Issue number | 3 |
| Publication status | Published - Jan 2017 |
Abstract
The use of spectroscopic sensors for bioprocess monitoring is a powerful tool within the process analytical technology (PAT) initiative of the US Food and Drug Administration. Spectroscopic sensors enable the simultaneous real-time bioprocess monitoring of various critical process parameters including biological, chemical, and physical variables during the entire biotechnological production process. This potential can be realized through the combination of spectroscopic measurements (UV/Vis spectroscopy, IR spectroscopy, fluorescence spectroscopy, and Raman spectroscopy) with multivariate data analysis to obtain relevant process information out of an enormous amount of data. This review summarizes the newest results from science and industry after the establishment of the PAT initiative and gives a critical overview of the most common in-line spectroscopic techniques. Examples are provided of the wide range of possible applications in upstream processing and downstream processing of spectroscopic sensors for real-time monitoring to optimize productivity and ensure product quality in the pharmaceutical industry.
Keywords
- Bioprocess monitoring, Critical process parameters, In-line monitoring, Process analytical technology, Spectroscopic sensors
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
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In: Analytical and Bioanalytical Chemistry, Vol. 409, No. 3, 01.2017, p. 651-666.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application
AU - Claßen, Jens
AU - Aupert, Florian
AU - Reardon, Kenneth F.
AU - Solle, Dörte
AU - Scheper, Thomas
N1 - Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2017/1
Y1 - 2017/1
N2 - The use of spectroscopic sensors for bioprocess monitoring is a powerful tool within the process analytical technology (PAT) initiative of the US Food and Drug Administration. Spectroscopic sensors enable the simultaneous real-time bioprocess monitoring of various critical process parameters including biological, chemical, and physical variables during the entire biotechnological production process. This potential can be realized through the combination of spectroscopic measurements (UV/Vis spectroscopy, IR spectroscopy, fluorescence spectroscopy, and Raman spectroscopy) with multivariate data analysis to obtain relevant process information out of an enormous amount of data. This review summarizes the newest results from science and industry after the establishment of the PAT initiative and gives a critical overview of the most common in-line spectroscopic techniques. Examples are provided of the wide range of possible applications in upstream processing and downstream processing of spectroscopic sensors for real-time monitoring to optimize productivity and ensure product quality in the pharmaceutical industry.
AB - The use of spectroscopic sensors for bioprocess monitoring is a powerful tool within the process analytical technology (PAT) initiative of the US Food and Drug Administration. Spectroscopic sensors enable the simultaneous real-time bioprocess monitoring of various critical process parameters including biological, chemical, and physical variables during the entire biotechnological production process. This potential can be realized through the combination of spectroscopic measurements (UV/Vis spectroscopy, IR spectroscopy, fluorescence spectroscopy, and Raman spectroscopy) with multivariate data analysis to obtain relevant process information out of an enormous amount of data. This review summarizes the newest results from science and industry after the establishment of the PAT initiative and gives a critical overview of the most common in-line spectroscopic techniques. Examples are provided of the wide range of possible applications in upstream processing and downstream processing of spectroscopic sensors for real-time monitoring to optimize productivity and ensure product quality in the pharmaceutical industry.
KW - Bioprocess monitoring
KW - Critical process parameters
KW - In-line monitoring
KW - Process analytical technology
KW - Spectroscopic sensors
UR - http://www.scopus.com/inward/record.url?scp=85000613582&partnerID=8YFLogxK
U2 - 10.1007/s00216-016-0068-x
DO - 10.1007/s00216-016-0068-x
M3 - Review article
C2 - 27900421
AN - SCOPUS:85000613582
VL - 409
SP - 651
EP - 666
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 3
ER -