Details
| Original language | English |
|---|---|
| Pages (from-to) | 405-416 |
| Number of pages | 12 |
| Journal | Engineering in life sciences |
| Volume | 16 |
| Issue number | 5 |
| Early online date | 2 Mar 2016 |
| Publication status | Published - 15 Jul 2016 |
Abstract
The number and use of automated cell culture systems for mammalian cell culture are steadily increasing. Automated cell culture systems require miniaturized analytics with a high throughput to obtain as much information as possible from single experiments. Standard analytics commonly used for conventional bioreactor samples cannot handle the high throughput and the low sample volumes. Spectroscopic methods provide a means of meeting this analytical requirement and afford fast and direct access to process information. In the first part of this review, UV/VIS, fluorescence, Raman, near-infrared, and mid-infrared spectroscopy are presented. In the second part of the review, these spectroscopic methods are evaluated in terms of their applicability in the new field of mammalian cell culture processes in automated cell culture systems. Unlike standard bioreactors, these automated systems have special requirements that apply to the use of spectroscopic methods. Therefore, they are compared with regard to cell culture automation, throughput, and required sample volume.
Keywords
- Automated small-scale cell culture system, High throughput, Mammalian cell culture, Process analytical technology, Spectroscopy
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
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In: Engineering in life sciences, Vol. 16, No. 5, 15.07.2016, p. 405-416.
Research output: Contribution to journal › Article › Research
}
TY - JOUR
T1 - Spectroscopic methods and their applicability for high-throughput characterization of mammalian cell cultures in automated cell culture systems
AU - Musmann, Carsten
AU - Joeris, Klaus
AU - Markert, Sven
AU - Solle, Dörte
AU - Scheper, Thomas
PY - 2016/7/15
Y1 - 2016/7/15
N2 - The number and use of automated cell culture systems for mammalian cell culture are steadily increasing. Automated cell culture systems require miniaturized analytics with a high throughput to obtain as much information as possible from single experiments. Standard analytics commonly used for conventional bioreactor samples cannot handle the high throughput and the low sample volumes. Spectroscopic methods provide a means of meeting this analytical requirement and afford fast and direct access to process information. In the first part of this review, UV/VIS, fluorescence, Raman, near-infrared, and mid-infrared spectroscopy are presented. In the second part of the review, these spectroscopic methods are evaluated in terms of their applicability in the new field of mammalian cell culture processes in automated cell culture systems. Unlike standard bioreactors, these automated systems have special requirements that apply to the use of spectroscopic methods. Therefore, they are compared with regard to cell culture automation, throughput, and required sample volume.
AB - The number and use of automated cell culture systems for mammalian cell culture are steadily increasing. Automated cell culture systems require miniaturized analytics with a high throughput to obtain as much information as possible from single experiments. Standard analytics commonly used for conventional bioreactor samples cannot handle the high throughput and the low sample volumes. Spectroscopic methods provide a means of meeting this analytical requirement and afford fast and direct access to process information. In the first part of this review, UV/VIS, fluorescence, Raman, near-infrared, and mid-infrared spectroscopy are presented. In the second part of the review, these spectroscopic methods are evaluated in terms of their applicability in the new field of mammalian cell culture processes in automated cell culture systems. Unlike standard bioreactors, these automated systems have special requirements that apply to the use of spectroscopic methods. Therefore, they are compared with regard to cell culture automation, throughput, and required sample volume.
KW - Automated small-scale cell culture system
KW - High throughput
KW - Mammalian cell culture
KW - Process analytical technology
KW - Spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=84964425613&partnerID=8YFLogxK
U2 - 10.1002/elsc.201500122
DO - 10.1002/elsc.201500122
M3 - Article
AN - SCOPUS:84964425613
VL - 16
SP - 405
EP - 416
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 5
ER -