Details
| Original language | English |
|---|---|
| Article number | 084903 |
| Journal | AIP Advances |
| Volume | 5 |
| Issue number | 8 |
| Early online date | 12 Mar 2015 |
| Publication status | Published - Aug 2015 |
Abstract
Multimodal nonlinear microscopy allows imaging of highly ordered biological tissue due to spectral separation of nonlinear signals. This requires certain knowledge about the spectral distribution of the different nonlinear signals. In contrast to several publications we demonstrate a factor of √2 relating the full width at half maximum of a gaussian laser pulse spectrum to the corresponding second harmonic pulse spectrum in the spatial domain by using a simple theoretical model. Experiments on monopotassium phosphate crystals (KDP-crystals) and on porcine corneal tissue support our theoretical predictions. Furthermore, no differences in spectral width were found for epi- and trans-detection of the second harmonic signal. Overall, these results may help to build an optimized multiphoton setup for spectral separation of nonlinear signals.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: AIP Advances, Vol. 5, No. 8, 084903, 08.2015.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Spectral behavior of second harmonic signals from organic and non-organic materials in multiphoton microscopy
AU - Ehmke, Tobias
AU - Knebl, Andreas
AU - Reiss, Stephan
AU - Fischinger, Isaak R.
AU - Seiler, Theo G.
AU - Stachs, Oliver
AU - Heisterkamp, Alexander
PY - 2015/8
Y1 - 2015/8
N2 - Multimodal nonlinear microscopy allows imaging of highly ordered biological tissue due to spectral separation of nonlinear signals. This requires certain knowledge about the spectral distribution of the different nonlinear signals. In contrast to several publications we demonstrate a factor of √2 relating the full width at half maximum of a gaussian laser pulse spectrum to the corresponding second harmonic pulse spectrum in the spatial domain by using a simple theoretical model. Experiments on monopotassium phosphate crystals (KDP-crystals) and on porcine corneal tissue support our theoretical predictions. Furthermore, no differences in spectral width were found for epi- and trans-detection of the second harmonic signal. Overall, these results may help to build an optimized multiphoton setup for spectral separation of nonlinear signals.
AB - Multimodal nonlinear microscopy allows imaging of highly ordered biological tissue due to spectral separation of nonlinear signals. This requires certain knowledge about the spectral distribution of the different nonlinear signals. In contrast to several publications we demonstrate a factor of √2 relating the full width at half maximum of a gaussian laser pulse spectrum to the corresponding second harmonic pulse spectrum in the spatial domain by using a simple theoretical model. Experiments on monopotassium phosphate crystals (KDP-crystals) and on porcine corneal tissue support our theoretical predictions. Furthermore, no differences in spectral width were found for epi- and trans-detection of the second harmonic signal. Overall, these results may help to build an optimized multiphoton setup for spectral separation of nonlinear signals.
UR - http://www.scopus.com/inward/record.url?scp=84924787208&partnerID=8YFLogxK
UR - https://aip.scitation.org/doi/10.1063/1.4931957
U2 - 10.1063/1.4915134
DO - 10.1063/1.4915134
M3 - Article
AN - SCOPUS:84924787208
VL - 5
JO - AIP Advances
JF - AIP Advances
SN - 2158-3226
IS - 8
M1 - 084903
ER -