Details
| Original language | English |
|---|---|
| Pages (from-to) | 1215-1221 |
| Number of pages | 7 |
| Journal | PHYTOPATHOLOGY |
| Volume | 86 |
| Issue number | 11 |
| Publication status | Published - Nov 1996 |
Abstract
Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.
Keywords
- detection, differentiation, insect transmission, nucleotide sequence of coat protein, phylogeny
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PHYTOPATHOLOGY, Vol. 86, No. 11, 11.1996, p. 1215-1221.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Sour cherry strain of plum pox potyvirus (PPV)
T2 - Molecular and serological evidence for a new subgroup of PPV strains
AU - Nemchinov, L.
AU - Hadidi, A.
AU - Maiss, E.
AU - Cambra, M.
AU - Candresse, T.
AU - Damsteegt, V.
PY - 1996/11
Y1 - 1996/11
N2 - Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.
AB - Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.
KW - detection
KW - differentiation
KW - insect transmission
KW - nucleotide sequence of coat protein
KW - phylogeny
UR - http://www.scopus.com/inward/record.url?scp=0029802736&partnerID=8YFLogxK
U2 - 10.1094/Phyto-86-1215
DO - 10.1094/Phyto-86-1215
M3 - Article
AN - SCOPUS:0029802736
VL - 86
SP - 1215
EP - 1221
JO - PHYTOPATHOLOGY
JF - PHYTOPATHOLOGY
SN - 0031-949X
IS - 11
ER -