Details
| Original language | English |
|---|---|
| Title of host publication | III International Symposium on Rose Research and Cultivation |
| Publisher | International Society for Horticultural Science |
| Pages | 341-347 |
| Number of pages | 7 |
| ISBN (print) | 9789066057746 |
| Publication status | Published - 2001 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 547 |
| ISSN (Print) | 0567-7572 |
Abstract
Somatic embryogenic callus was initiated from in vitro derived leaf explants following an induction period of four weeks on MS basal medium supplemented with 2,4-D or NAA and several further subcultures on MS medium with zeatine or TDZ. The embryogenic callus was propagated on MS medium containing the phytohormones NAA, zeatine and GA3. For the cultivars 'Heckenzauber' and 'Pariser Charme' long-term-cultures as well as cell suspensions could be established. Incubation in a 16 h photoperiod was essential for the induction of somatic embryogenesis as well as for callus and embryo development. Although the regeneration protocol originally was developed for the cultivars 'Heckenzauber' and 'Pariser Charme', it could be adapted to a broad range of further rose genotypes. Up to now, from 50 tested genotypes 69 % could be regenerated. A critical point in the regeneration process is development of the embryos to plants. Germination occurs only sporadically and the somatic embryos have to be regenerated via adventitious shoot formation. The analysis of about 500 regenerated plants from different cultivars for several morphological traits revealed, that nearly all regenerants were true-to-type.
Keywords
- Adventitious shoot formation, AFLP marker, Callus culture, Cell suspension culture, Mass propagation, Somaclonal variation, Transformation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
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III International Symposium on Rose Research and Cultivation. International Society for Horticultural Science, 2001. p. 341-347 (Acta Horticulturae; Vol. 547).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Somatic embryogenesis in roses
AU - Dohm, Andrea
AU - Ludwig, Clarisse
AU - Nehring, Kristin
AU - Debener, Thomas
PY - 2001
Y1 - 2001
N2 - Somatic embryogenic callus was initiated from in vitro derived leaf explants following an induction period of four weeks on MS basal medium supplemented with 2,4-D or NAA and several further subcultures on MS medium with zeatine or TDZ. The embryogenic callus was propagated on MS medium containing the phytohormones NAA, zeatine and GA3. For the cultivars 'Heckenzauber' and 'Pariser Charme' long-term-cultures as well as cell suspensions could be established. Incubation in a 16 h photoperiod was essential for the induction of somatic embryogenesis as well as for callus and embryo development. Although the regeneration protocol originally was developed for the cultivars 'Heckenzauber' and 'Pariser Charme', it could be adapted to a broad range of further rose genotypes. Up to now, from 50 tested genotypes 69 % could be regenerated. A critical point in the regeneration process is development of the embryos to plants. Germination occurs only sporadically and the somatic embryos have to be regenerated via adventitious shoot formation. The analysis of about 500 regenerated plants from different cultivars for several morphological traits revealed, that nearly all regenerants were true-to-type.
AB - Somatic embryogenic callus was initiated from in vitro derived leaf explants following an induction period of four weeks on MS basal medium supplemented with 2,4-D or NAA and several further subcultures on MS medium with zeatine or TDZ. The embryogenic callus was propagated on MS medium containing the phytohormones NAA, zeatine and GA3. For the cultivars 'Heckenzauber' and 'Pariser Charme' long-term-cultures as well as cell suspensions could be established. Incubation in a 16 h photoperiod was essential for the induction of somatic embryogenesis as well as for callus and embryo development. Although the regeneration protocol originally was developed for the cultivars 'Heckenzauber' and 'Pariser Charme', it could be adapted to a broad range of further rose genotypes. Up to now, from 50 tested genotypes 69 % could be regenerated. A critical point in the regeneration process is development of the embryos to plants. Germination occurs only sporadically and the somatic embryos have to be regenerated via adventitious shoot formation. The analysis of about 500 regenerated plants from different cultivars for several morphological traits revealed, that nearly all regenerants were true-to-type.
KW - Adventitious shoot formation
KW - AFLP marker
KW - Callus culture
KW - Cell suspension culture
KW - Mass propagation
KW - Somaclonal variation
KW - Transformation
UR - http://www.scopus.com/inward/record.url?scp=0042437682&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2001.547.40
DO - 10.17660/ActaHortic.2001.547.40
M3 - Contribution to book/anthology
AN - SCOPUS:0042437682
SN - 9789066057746
T3 - Acta Horticulturae
SP - 341
EP - 347
BT - III International Symposium on Rose Research and Cultivation
PB - International Society for Horticultural Science
ER -