Details
| Original language | English |
|---|---|
| Pages (from-to) | 1855-1863 |
| Number of pages | 9 |
| Journal | Biotechnology and Bioengineering |
| Volume | 109 |
| Issue number | 7 |
| Publication status | Published - 27 Jan 2012 |
| Externally published | Yes |
Abstract
For the aim of ex vivo engineering of functional tissue substitutes, Laser-assisted BioPrinting (LaBP) is under investigation for the arrangement of living cells in predefined patterns. So far three-dimensional (3D) arrangements of single or two-dimensional (2D) patterning of different cell types have been presented. It has been shown that cells are not harmed by the printing procedure. We now demonstrate for the first time the 3D arrangement of vital cells by LaBP as multicellular grafts analogous to native archetype and the formation of tissue by these cells. For this purpose, fibroblasts and keratinocytes embedded in collagen were printed in 3D as a simple example for skin tissue. To study cell functions and tissue formation process in 3D, different characteristics, such as cell localisation and proliferation were investigated. We further analysed the formation of adhering and gap junctions, which are fundamental for tissue morphogenesis and cohesion. In this study, it was demonstrated that LaBP is an outstanding tool for the generation of multicellular 3D constructs mimicking tissue functions. These findings are promising for the realisation of 3D in vitro models and tissue substitutes for many applications in tissue engineering.
Keywords
- 3D tissue generation, Bioprinting, Cell printing, Laser, Skin
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Biotechnology and Bioengineering, Vol. 109, No. 7, 27.01.2012, p. 1855-1863.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Skin Tissue Generation by Laser Cell Printing
AU - Koch, Lothar
AU - Deiwick, Andrea
AU - Schlie, Sabrina
AU - Michael, Stefanie
AU - Gruene, Martin
AU - Coger, Vincent
AU - Zychlinski, Daniela
AU - Schambach, Axel
AU - Reimers, Kerstin
AU - Vogt, Peter M.
AU - Chichkov, Boris
PY - 2012/1/27
Y1 - 2012/1/27
N2 - For the aim of ex vivo engineering of functional tissue substitutes, Laser-assisted BioPrinting (LaBP) is under investigation for the arrangement of living cells in predefined patterns. So far three-dimensional (3D) arrangements of single or two-dimensional (2D) patterning of different cell types have been presented. It has been shown that cells are not harmed by the printing procedure. We now demonstrate for the first time the 3D arrangement of vital cells by LaBP as multicellular grafts analogous to native archetype and the formation of tissue by these cells. For this purpose, fibroblasts and keratinocytes embedded in collagen were printed in 3D as a simple example for skin tissue. To study cell functions and tissue formation process in 3D, different characteristics, such as cell localisation and proliferation were investigated. We further analysed the formation of adhering and gap junctions, which are fundamental for tissue morphogenesis and cohesion. In this study, it was demonstrated that LaBP is an outstanding tool for the generation of multicellular 3D constructs mimicking tissue functions. These findings are promising for the realisation of 3D in vitro models and tissue substitutes for many applications in tissue engineering.
AB - For the aim of ex vivo engineering of functional tissue substitutes, Laser-assisted BioPrinting (LaBP) is under investigation for the arrangement of living cells in predefined patterns. So far three-dimensional (3D) arrangements of single or two-dimensional (2D) patterning of different cell types have been presented. It has been shown that cells are not harmed by the printing procedure. We now demonstrate for the first time the 3D arrangement of vital cells by LaBP as multicellular grafts analogous to native archetype and the formation of tissue by these cells. For this purpose, fibroblasts and keratinocytes embedded in collagen were printed in 3D as a simple example for skin tissue. To study cell functions and tissue formation process in 3D, different characteristics, such as cell localisation and proliferation were investigated. We further analysed the formation of adhering and gap junctions, which are fundamental for tissue morphogenesis and cohesion. In this study, it was demonstrated that LaBP is an outstanding tool for the generation of multicellular 3D constructs mimicking tissue functions. These findings are promising for the realisation of 3D in vitro models and tissue substitutes for many applications in tissue engineering.
KW - 3D tissue generation
KW - Bioprinting
KW - Cell printing
KW - Laser
KW - Skin
UR - http://www.scopus.com/inward/record.url?scp=84861199493&partnerID=8YFLogxK
U2 - 10.1002/bit.24455
DO - 10.1002/bit.24455
M3 - Article
C2 - 22328297
AN - SCOPUS:84861199493
VL - 109
SP - 1855
EP - 1863
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 7
ER -