Details
| Original language | English |
|---|---|
| Pages (from-to) | 266-274 |
| Number of pages | 9 |
| Journal | Journal of Food Composition and Analysis |
| Volume | 31 |
| Issue number | 2 |
| Publication status | Published - 30 Aug 2013 |
Abstract
A single-run high performance liquid chromatography (HPLC) with diode array detector (DAD) based method was developed for the separation, identification and comprehensive quantification of degradation products of ergosterol formed in oyster mushroom (Pleurotus ostreatus) after UV-B exposure. After 60min, 10 substances involved in the photoprocess were separated, identified by their characteristic DAD spectrum and distinguished by their molecular weight, in cases where spectra were identical: vitamin D2, previtamin D2, tachysterol2, lumisterol2 and ergosterol, and, in minor quantity, their structural analogues of the 22,23-dihydroergocalciferol (vitamin D4) series. Sample preparation protocol affected the total yields and the ratios of previtamin and vitamin D2/D4. Hot alkaline hydrolysis resulted in the best digestion of the mushroom matrix and accordingly gave the highest vitamin D yield (D2: 141.32μgg-1 dry matter, DM; D4: 22.72μgg-1 DM). Limit of detection for vitamin D2/4 was 0.02μgg-1 dry matter (DM) and was estimated for previtamin D2/4 (0.06μgg-1 DM), tachysterol2/4 (0.02μgg-1 DM) and lumisterol2/4 (0.06μgg-1 DM). Recovery of spiked vitamin D2 was 97±0.7%. The study provides an analytical tool to assess the process of vitamin D generation after UV-B treatment for the production of oyster mushrooms with a balanced nutritional profile of vitamin D compounds.
Keywords
- 22,23-Dihydroergocalciferol, Ergocalciferol (vitamin D), Food analysis, Food composition, Lumisterol, Photoisomers, Pleurotus ostreatus, Previtamin D, Tachysterol, Vitamin D activity, Vitamin D
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
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In: Journal of Food Composition and Analysis, Vol. 31, No. 2, 30.08.2013, p. 266-274.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Single-run analysis of vitamin D photoproducts in oyster mushroom (Pleurotus ostreatus) after UV-B treatment
AU - Wittig, Maximilian
AU - Krings, Ulrich
AU - Berger, Ralf G.
N1 - Funding information: The authors wish to express their thanks to Martin Rühl, Druid Austernpilze, for providing fresh and certified mushroom material. This project was supported by the Lower Saxony Ministry of Science and Arts , FAEN project #6.
PY - 2013/8/30
Y1 - 2013/8/30
N2 - A single-run high performance liquid chromatography (HPLC) with diode array detector (DAD) based method was developed for the separation, identification and comprehensive quantification of degradation products of ergosterol formed in oyster mushroom (Pleurotus ostreatus) after UV-B exposure. After 60min, 10 substances involved in the photoprocess were separated, identified by their characteristic DAD spectrum and distinguished by their molecular weight, in cases where spectra were identical: vitamin D2, previtamin D2, tachysterol2, lumisterol2 and ergosterol, and, in minor quantity, their structural analogues of the 22,23-dihydroergocalciferol (vitamin D4) series. Sample preparation protocol affected the total yields and the ratios of previtamin and vitamin D2/D4. Hot alkaline hydrolysis resulted in the best digestion of the mushroom matrix and accordingly gave the highest vitamin D yield (D2: 141.32μgg-1 dry matter, DM; D4: 22.72μgg-1 DM). Limit of detection for vitamin D2/4 was 0.02μgg-1 dry matter (DM) and was estimated for previtamin D2/4 (0.06μgg-1 DM), tachysterol2/4 (0.02μgg-1 DM) and lumisterol2/4 (0.06μgg-1 DM). Recovery of spiked vitamin D2 was 97±0.7%. The study provides an analytical tool to assess the process of vitamin D generation after UV-B treatment for the production of oyster mushrooms with a balanced nutritional profile of vitamin D compounds.
AB - A single-run high performance liquid chromatography (HPLC) with diode array detector (DAD) based method was developed for the separation, identification and comprehensive quantification of degradation products of ergosterol formed in oyster mushroom (Pleurotus ostreatus) after UV-B exposure. After 60min, 10 substances involved in the photoprocess were separated, identified by their characteristic DAD spectrum and distinguished by their molecular weight, in cases where spectra were identical: vitamin D2, previtamin D2, tachysterol2, lumisterol2 and ergosterol, and, in minor quantity, their structural analogues of the 22,23-dihydroergocalciferol (vitamin D4) series. Sample preparation protocol affected the total yields and the ratios of previtamin and vitamin D2/D4. Hot alkaline hydrolysis resulted in the best digestion of the mushroom matrix and accordingly gave the highest vitamin D yield (D2: 141.32μgg-1 dry matter, DM; D4: 22.72μgg-1 DM). Limit of detection for vitamin D2/4 was 0.02μgg-1 dry matter (DM) and was estimated for previtamin D2/4 (0.06μgg-1 DM), tachysterol2/4 (0.02μgg-1 DM) and lumisterol2/4 (0.06μgg-1 DM). Recovery of spiked vitamin D2 was 97±0.7%. The study provides an analytical tool to assess the process of vitamin D generation after UV-B treatment for the production of oyster mushrooms with a balanced nutritional profile of vitamin D compounds.
KW - 22,23-Dihydroergocalciferol
KW - Ergocalciferol (vitamin D)
KW - Food analysis
KW - Food composition
KW - Lumisterol
KW - Photoisomers
KW - Pleurotus ostreatus
KW - Previtamin D
KW - Tachysterol
KW - Vitamin D activity
KW - Vitamin D
UR - http://www.scopus.com/inward/record.url?scp=84886239093&partnerID=8YFLogxK
U2 - 10.1016/j.jfca.2013.05.017
DO - 10.1016/j.jfca.2013.05.017
M3 - Article
AN - SCOPUS:84886239093
VL - 31
SP - 266
EP - 274
JO - Journal of Food Composition and Analysis
JF - Journal of Food Composition and Analysis
SN - 0889-1575
IS - 2
ER -