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Single Molecule DNA Detection with an Atomic Vapor Notch Filter

Research output: Contribution to journal › Article › Research › peer review

Authors

  • Denis Uhland
  • Torsten Rendler
  • Matthias Widmann
  • Sang-Yun Lee
  • Ilja Gerhardt

Research Organisations

Details

Original languageEnglish
Pages (from-to)20
Number of pages1
JournalEPJ Quantum Technology
Volume2
Publication statusPublished - 2015

Abstract

The detection of single molecules has facilitated many advances in life- and material-science. Commonly the fluorescence of dye molecules is detected, which are attached to a non-fluorescent structure under study. For fluorescence microscopy one desires to maximize the detection efficiency together with an efficient suppression of undesired laser leakage. Here we present the use of the narrow-band filtering properties of hot atomic sodium vapor to selectively filter the excitation light from the red-shifted fluorescence of dye labeled single-stranded DNA molecules. A statistical analysis proves an enhancement in detection efficiency of more than 15field configuration.

Keywords

    Single Molecule Detection, Atomic Filters, Sodium spectroscopy, DNA detection

Cite this

Single Molecule DNA Detection with an Atomic Vapor Notch Filter. / Uhland, Denis; Rendler, Torsten; Widmann, Matthias et al.
In: EPJ Quantum Technology, Vol. 2, 2015, p. 20.

Research output: Contribution to journal › Article › Research › peer review

Uhland, D, Rendler, T, Widmann, M, Lee, S-Y, Wrachtrup, J & Gerhardt, I 2015, 'Single Molecule DNA Detection with an Atomic Vapor Notch Filter', EPJ Quantum Technology, vol. 2, pp. 20. <http://www.epjquantumtechnology.com/content/2/1/20>
Uhland, D., Rendler, T., Widmann, M., Lee, S.-Y., Wrachtrup, J., & Gerhardt, I. (2015). Single Molecule DNA Detection with an Atomic Vapor Notch Filter. EPJ Quantum Technology, 2, 20. http://www.epjquantumtechnology.com/content/2/1/20
Uhland D, Rendler T, Widmann M, Lee SY, Wrachtrup J, Gerhardt I. Single Molecule DNA Detection with an Atomic Vapor Notch Filter. EPJ Quantum Technology. 2015;2:20.
Uhland, Denis ; Rendler, Torsten ; Widmann, Matthias et al. / Single Molecule DNA Detection with an Atomic Vapor Notch Filter. In: EPJ Quantum Technology. 2015 ; Vol. 2. pp. 20.
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title = "Single Molecule DNA Detection with an Atomic Vapor Notch Filter",
abstract = "The detection of single molecules has facilitated many advances in life- and material-science. Commonly the fluorescence of dye molecules is detected, which are attached to a non-fluorescent structure under study. For fluorescence microscopy one desires to maximize the detection efficiency together with an efficient suppression of undesired laser leakage. Here we present the use of the narrow-band filtering properties of hot atomic sodium vapor to selectively filter the excitation light from the red-shifted fluorescence of dye labeled single-stranded DNA molecules. A statistical analysis proves an enhancement in detection efficiency of more than 15field configuration.",
keywords = "Single Molecule Detection, Atomic Filters, Sodium spectroscopy, DNA detection",
author = "Denis Uhland and Torsten Rendler and Matthias Widmann and Sang-Yun Lee and J{\"o}rg Wrachtrup and Ilja Gerhardt",
note = "Funding information: We thank Andrea Zappe for DNA preparation and Helmut Kammerlander from the glass shop for producing several sodium cells. We also thank Philip Hemmer and Nathan Chejanovsky for carefully proofreading the manuscript. Initial experiments were conducted by Guilherme Stein & Petr Siyushev. We acknowledge funding from the Max Planck Society (JW, Max Planck fellowship).",
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Download

TY - JOUR

T1 - Single Molecule DNA Detection with an Atomic Vapor Notch Filter

AU - Uhland, Denis

AU - Rendler, Torsten

AU - Widmann, Matthias

AU - Lee, Sang-Yun

AU - Wrachtrup, Jörg

AU - Gerhardt, Ilja

N1 - Funding information: We thank Andrea Zappe for DNA preparation and Helmut Kammerlander from the glass shop for producing several sodium cells. We also thank Philip Hemmer and Nathan Chejanovsky for carefully proofreading the manuscript. Initial experiments were conducted by Guilherme Stein & Petr Siyushev. We acknowledge funding from the Max Planck Society (JW, Max Planck fellowship).

PY - 2015

Y1 - 2015

N2 - The detection of single molecules has facilitated many advances in life- and material-science. Commonly the fluorescence of dye molecules is detected, which are attached to a non-fluorescent structure under study. For fluorescence microscopy one desires to maximize the detection efficiency together with an efficient suppression of undesired laser leakage. Here we present the use of the narrow-band filtering properties of hot atomic sodium vapor to selectively filter the excitation light from the red-shifted fluorescence of dye labeled single-stranded DNA molecules. A statistical analysis proves an enhancement in detection efficiency of more than 15field configuration.

AB - The detection of single molecules has facilitated many advances in life- and material-science. Commonly the fluorescence of dye molecules is detected, which are attached to a non-fluorescent structure under study. For fluorescence microscopy one desires to maximize the detection efficiency together with an efficient suppression of undesired laser leakage. Here we present the use of the narrow-band filtering properties of hot atomic sodium vapor to selectively filter the excitation light from the red-shifted fluorescence of dye labeled single-stranded DNA molecules. A statistical analysis proves an enhancement in detection efficiency of more than 15field configuration.

KW - Single Molecule Detection

KW - Atomic Filters

KW - Sodium spectroscopy

KW - DNA detection

M3 - Article

VL - 2

SP - 20

JO - EPJ Quantum Technology

JF - EPJ Quantum Technology

ER -

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