Simple technique for simultaneous on-line estimation of biomass and acetate from base consumption and conductivity measurements in high-cell density cultures of Escherichia coli

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Frank Hoffmann
  • Michael Schmidt
  • Ursula Rinas

External Research Organisations

  • Helmholtz Centre for Infection Research (HZI)
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Details

Original languageEnglish
Pages (from-to)358-361
Number of pages4
JournalBiotechnology and bioengineering
Volume70
Issue number3
Publication statusPublished - 12 Sept 2000
Externally publishedYes

Abstract

In high-cell density cultures of Escherichia coli, the amount of ammonia required to maintain a constant pH was an effective on-line measure of biomass. When acetic acid formation occurred, biomass estimates were corrected by additional measurements of the culture conductivity. In the absence of acetic acid excretion, the culture conductivity decreased approximately linearly during the fed-batch phase of the high-cell density cultivation. Upward deviation of the culture conductivity from this trend was proportional to the acetate concentration in the medium. Thus, simultaneous determination of the acetic acid concentration and correction of biomass estimates from ammonia consumption were possible by conductivity measurements. (C) 2000 John Wiley and Sons, Inc.

Keywords

    Acetate, Biomass, Estimation, On-line

ASJC Scopus subject areas

Cite this

Simple technique for simultaneous on-line estimation of biomass and acetate from base consumption and conductivity measurements in high-cell density cultures of Escherichia coli. / Hoffmann, Frank; Schmidt, Michael; Rinas, Ursula.
In: Biotechnology and bioengineering, Vol. 70, No. 3, 12.09.2000, p. 358-361.

Research output: Contribution to journalArticleResearchpeer review

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AU - Rinas, Ursula

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