Details
| Original language | English |
|---|---|
| Pages (from-to) | 59-65 |
| Number of pages | 7 |
| Journal | Journal of biotechnology |
| Volume | 39 |
| Issue number | 1 |
| Publication status | Published - 21 Feb 1995 |
| Externally published | Yes |
Abstract
A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (μ < μcrit). Cell concentrations of 128 and 148 g per 1 dry cell weight (g 1-1 DCW) were obtained using glucose or glycerol as carbon source, respectively.
Keywords
- Cultivation, high cell density, Escherichia coli, Fed-batch
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 39, No. 1, 21.02.1995, p. 59-65.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Simple fed-batch technique for high cell density cultivation of Escherichia coli
AU - Korz, D. J.
AU - Rinas, U.
AU - Hellmuth, K.
AU - Sanders, E. A.
AU - Deckwer, W. D.
N1 - Funding Information: We gratefullya cknowledget he excellentt ech-nical assistanceo f Martina Schreiner.T his work was supportedi n part by the Fonds der Chemi-schenI ndustrie (to UR).
PY - 1995/2/21
Y1 - 1995/2/21
N2 - A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (μ < μcrit). Cell concentrations of 128 and 148 g per 1 dry cell weight (g 1-1 DCW) were obtained using glucose or glycerol as carbon source, respectively.
AB - A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (μ < μcrit). Cell concentrations of 128 and 148 g per 1 dry cell weight (g 1-1 DCW) were obtained using glucose or glycerol as carbon source, respectively.
KW - Cultivation, high cell density
KW - Escherichia coli
KW - Fed-batch
UR - http://www.scopus.com/inward/record.url?scp=0029239706&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(94)00143-Z
DO - 10.1016/0168-1656(94)00143-Z
M3 - Article
C2 - 7766011
AN - SCOPUS:0029239706
VL - 39
SP - 59
EP - 65
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 1
ER -