Details
| Original language | English |
|---|---|
| Pages (from-to) | 2001-11 |
| Number of pages | 11 |
| Journal | Journal of experimental botany |
| Volume | 62 |
| Issue number | 6 |
| Publication status | Published - Mar 2011 |
Abstract
The beneficial element silicon (Si) may affect radial oxygen loss (ROL) of rice roots depending on suberization of the exodermis and lignification of sclerenchyma. Thus, the effect of Si nutrition on the oxidation power of rice roots, suberization and lignification was examined. In addition, Si-induced alterations of the transcript levels of 265 genes related to suberin and lignin synthesis were studied by custom-made microarray and quantitative Real Time-PCR. Without Si supply, the oxidation zone of 12 cm long adventitious roots extended along the entire root length but with Si supply the oxidation zone was restricted to 5 cm behind the root tip. This pattern coincided with enhanced suberization of the exodermis and lignification of sclerenchyma by Si supply. Suberization of the exodermis started, with and without Si supply, at 4-5 cm and 8-9 cm distance from the root tip (drt), respectively. Si significantly increased transcript abundance of 12 genes, while two genes had a reduced transcript level. A gene coding for a leucine-rich repeat protein exhibited a 25-fold higher transcript level with Si nutrition. Physiological, histochemical, and molecular-biological data showing that Si has an active impact on rice root anatomy and gene transcription is presented here.
Keywords
- Gene Expression Profiling, Gene Expression Regulation, Plant, Lignin/metabolism, Lipids/physiology, Oryza/growth & development, Oxidation-Reduction, Plant Roots/growth & development, Silicic Acid/metabolism, Silicon/metabolism
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In: Journal of experimental botany, Vol. 62, No. 6, 03.2011, p. 2001-11.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Silicon enhances suberization and lignification in roots of rice (Oryza sativa)
AU - Fleck, Alexander T
AU - Nye, Thandar
AU - Repenning, Cornelia
AU - Stahl, Frank
AU - Zahn, Marc
AU - Schenk, Manfred K
PY - 2011/3
Y1 - 2011/3
N2 - The beneficial element silicon (Si) may affect radial oxygen loss (ROL) of rice roots depending on suberization of the exodermis and lignification of sclerenchyma. Thus, the effect of Si nutrition on the oxidation power of rice roots, suberization and lignification was examined. In addition, Si-induced alterations of the transcript levels of 265 genes related to suberin and lignin synthesis were studied by custom-made microarray and quantitative Real Time-PCR. Without Si supply, the oxidation zone of 12 cm long adventitious roots extended along the entire root length but with Si supply the oxidation zone was restricted to 5 cm behind the root tip. This pattern coincided with enhanced suberization of the exodermis and lignification of sclerenchyma by Si supply. Suberization of the exodermis started, with and without Si supply, at 4-5 cm and 8-9 cm distance from the root tip (drt), respectively. Si significantly increased transcript abundance of 12 genes, while two genes had a reduced transcript level. A gene coding for a leucine-rich repeat protein exhibited a 25-fold higher transcript level with Si nutrition. Physiological, histochemical, and molecular-biological data showing that Si has an active impact on rice root anatomy and gene transcription is presented here.
AB - The beneficial element silicon (Si) may affect radial oxygen loss (ROL) of rice roots depending on suberization of the exodermis and lignification of sclerenchyma. Thus, the effect of Si nutrition on the oxidation power of rice roots, suberization and lignification was examined. In addition, Si-induced alterations of the transcript levels of 265 genes related to suberin and lignin synthesis were studied by custom-made microarray and quantitative Real Time-PCR. Without Si supply, the oxidation zone of 12 cm long adventitious roots extended along the entire root length but with Si supply the oxidation zone was restricted to 5 cm behind the root tip. This pattern coincided with enhanced suberization of the exodermis and lignification of sclerenchyma by Si supply. Suberization of the exodermis started, with and without Si supply, at 4-5 cm and 8-9 cm distance from the root tip (drt), respectively. Si significantly increased transcript abundance of 12 genes, while two genes had a reduced transcript level. A gene coding for a leucine-rich repeat protein exhibited a 25-fold higher transcript level with Si nutrition. Physiological, histochemical, and molecular-biological data showing that Si has an active impact on rice root anatomy and gene transcription is presented here.
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Plant
KW - Lignin/metabolism
KW - Lipids/physiology
KW - Oryza/growth & development
KW - Oxidation-Reduction
KW - Plant Roots/growth & development
KW - Silicic Acid/metabolism
KW - Silicon/metabolism
U2 - 10.1093/jxb/erq392
DO - 10.1093/jxb/erq392
M3 - Article
C2 - 21172812
VL - 62
SP - 2001
EP - 2011
JO - Journal of experimental botany
JF - Journal of experimental botany
SN - 0022-0957
IS - 6
ER -