Details
| Original language | English |
|---|---|
| Pages (from-to) | 85-90 |
| Number of pages | 6 |
| Journal | Scientia horticulturae |
| Volume | 93 |
| Issue number | 1 |
| Early online date | 23 Jan 2002 |
| Publication status | E-pub ahead of print - 23 Jan 2002 |
Abstract
Adult plants of Campanula carpatica Jacq. were obtained from shoots regenerated via callus derived from cotyledons of two cultivars: White Uniform and Blue Uniform. An optimized procedure used one medium, containing 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), NAA (α-naphthalene acetic acid) for producing both callus and shoots from White Uniform and Blue Uniform. Transfer of shoots to hormone-free medium induced the growth of roots. When roots had developed, the plantlets were transferred to soil, and developed into mature plants under greenhouse conditions. The highest achieved rate of explants resulting in mature plants was 2.8%. There were no significant differences in overall regeneration efficiency, i.e. number of explants forming rooted shoots between these two cultivars. A third cultivar, Blue Clips, produced a few shoots of which none formed roots. Attempts to regenerate plants from hypocotyls or young leaves were not successful.
Keywords
- Callus, Campanula, Regeneration
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Scientia horticulturae, Vol. 93, No. 1, 23.01.2002, p. 85-90.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Shoot regeneration of campanula carpatica jacq. (Campanulaceae) via callus phase
AU - Frello, Stefan
AU - Stummann, Bjarne Mejnert
AU - Serek, Margrethe
N1 - Funding Information: The authors thank Prof. Sven B. Andersen for general advice, Prof. Michael S. Reid and Dr. Sridevy Sriskandarajah for critical review of the manuscript. The project was funded by the Danish Ministry of Agriculture (93S-2466-Å97-01023) and Danish Campanula Growers: Gartneriet Thoruplund A/S (Odense), Gartneriet PKM ApS (Odense), Gartneriet Lumbyholm A/S (Odense), and Gartneriet Elmegaard ApS (Skælskør).
PY - 2002/1/23
Y1 - 2002/1/23
N2 - Adult plants of Campanula carpatica Jacq. were obtained from shoots regenerated via callus derived from cotyledons of two cultivars: White Uniform and Blue Uniform. An optimized procedure used one medium, containing 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), NAA (α-naphthalene acetic acid) for producing both callus and shoots from White Uniform and Blue Uniform. Transfer of shoots to hormone-free medium induced the growth of roots. When roots had developed, the plantlets were transferred to soil, and developed into mature plants under greenhouse conditions. The highest achieved rate of explants resulting in mature plants was 2.8%. There were no significant differences in overall regeneration efficiency, i.e. number of explants forming rooted shoots between these two cultivars. A third cultivar, Blue Clips, produced a few shoots of which none formed roots. Attempts to regenerate plants from hypocotyls or young leaves were not successful.
AB - Adult plants of Campanula carpatica Jacq. were obtained from shoots regenerated via callus derived from cotyledons of two cultivars: White Uniform and Blue Uniform. An optimized procedure used one medium, containing 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), NAA (α-naphthalene acetic acid) for producing both callus and shoots from White Uniform and Blue Uniform. Transfer of shoots to hormone-free medium induced the growth of roots. When roots had developed, the plantlets were transferred to soil, and developed into mature plants under greenhouse conditions. The highest achieved rate of explants resulting in mature plants was 2.8%. There were no significant differences in overall regeneration efficiency, i.e. number of explants forming rooted shoots between these two cultivars. A third cultivar, Blue Clips, produced a few shoots of which none formed roots. Attempts to regenerate plants from hypocotyls or young leaves were not successful.
KW - Callus
KW - Campanula
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=0037186401&partnerID=8YFLogxK
U2 - 10.1016/S0304-4238(01)00312-0
DO - 10.1016/S0304-4238(01)00312-0
M3 - Article
AN - SCOPUS:0037186401
VL - 93
SP - 85
EP - 90
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
IS - 1
ER -