Details
| Original language | English |
|---|---|
| Article number | 109578 |
| Journal | Soil Biology and Biochemistry |
| Volume | 198 |
| Early online date | 2 Sept 2024 |
| Publication status | E-pub ahead of print - 2 Sept 2024 |
Abstract
Decoding the fundamental taxa that decompose crop rhizodeposits (rhizo-C) and/or straw residue (straw-C) is crucial for understanding the role of plant-derived carbon (C) in driving microbial community assembly and consequent C decomposition. Here, a parallel 13C-labeling design, DNA-SIP, and metagenomics techniques were combined to separate maize rhizo-C utilizers from straw-C utilizers in agriculture soils containing both C sources. Also, by comparing bacterial utilizers and their C metabolisms in soils amended with a single C source (e.g., straw-13C only) and two C sources (e.g., straw-13C and rhizo-12C), we investigated the shift of composition and metabolisms of soil bacterial utilizers responding to C sources shift (e.g., compositional and metabolic changes of straw-13C utilizers from soil containing straw-13C to soil containing both straw-13C and rhizo-12C). We revealed i) Proteobacteria predominantly utilized rhizo-13C, while Firmicutes dominated the community specializing in straw-13C decomposition in soil containing both straw-C and rhizo-C; ii) the planted maize (i.e. rhizo-C input) changed community composition and metabolisms of straw-C utilizers, which shifted from K-strategists characterized by an enrichment of lignin-degrading genes to r-strategists which exhibited an enrichment of genes related to polysaccharide degradation. This metabolic shift of straw-C utilizer ultimately reduced straw-13C mineralization by 25.6% when maize was planted. This study identified the distinct utilizers of rhizo-C and straw-C in soils containing both C sources, and shed light on the shift of bacterial community and their metabolic activities responding to the changes of maize-derived C sources.
Keywords
- Agro-ecosystem, Bacterial community, Carbon metabolism, Continuous CO labeling, Rhizo-C/straw utilizers, SIP-Metagenome
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Soil Biology and Biochemistry, Vol. 198, 109578, 11.2024.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Shift of microbial taxa and metabolisms relying on carbon sources of rhizodeposits and straw of Zea mays L
AU - Fu, Yingyi
AU - Luo, Yu
AU - Qi, Jiejun
AU - He, Xinhua
AU - Zhang, Haoqing
AU - Guggenberger, Georg
AU - Xu, Jianming
N1 - Publisher Copyright: © 2024
PY - 2024/9/2
Y1 - 2024/9/2
N2 - Decoding the fundamental taxa that decompose crop rhizodeposits (rhizo-C) and/or straw residue (straw-C) is crucial for understanding the role of plant-derived carbon (C) in driving microbial community assembly and consequent C decomposition. Here, a parallel 13C-labeling design, DNA-SIP, and metagenomics techniques were combined to separate maize rhizo-C utilizers from straw-C utilizers in agriculture soils containing both C sources. Also, by comparing bacterial utilizers and their C metabolisms in soils amended with a single C source (e.g., straw-13C only) and two C sources (e.g., straw-13C and rhizo-12C), we investigated the shift of composition and metabolisms of soil bacterial utilizers responding to C sources shift (e.g., compositional and metabolic changes of straw-13C utilizers from soil containing straw-13C to soil containing both straw-13C and rhizo-12C). We revealed i) Proteobacteria predominantly utilized rhizo-13C, while Firmicutes dominated the community specializing in straw-13C decomposition in soil containing both straw-C and rhizo-C; ii) the planted maize (i.e. rhizo-C input) changed community composition and metabolisms of straw-C utilizers, which shifted from K-strategists characterized by an enrichment of lignin-degrading genes to r-strategists which exhibited an enrichment of genes related to polysaccharide degradation. This metabolic shift of straw-C utilizer ultimately reduced straw-13C mineralization by 25.6% when maize was planted. This study identified the distinct utilizers of rhizo-C and straw-C in soils containing both C sources, and shed light on the shift of bacterial community and their metabolic activities responding to the changes of maize-derived C sources.
AB - Decoding the fundamental taxa that decompose crop rhizodeposits (rhizo-C) and/or straw residue (straw-C) is crucial for understanding the role of plant-derived carbon (C) in driving microbial community assembly and consequent C decomposition. Here, a parallel 13C-labeling design, DNA-SIP, and metagenomics techniques were combined to separate maize rhizo-C utilizers from straw-C utilizers in agriculture soils containing both C sources. Also, by comparing bacterial utilizers and their C metabolisms in soils amended with a single C source (e.g., straw-13C only) and two C sources (e.g., straw-13C and rhizo-12C), we investigated the shift of composition and metabolisms of soil bacterial utilizers responding to C sources shift (e.g., compositional and metabolic changes of straw-13C utilizers from soil containing straw-13C to soil containing both straw-13C and rhizo-12C). We revealed i) Proteobacteria predominantly utilized rhizo-13C, while Firmicutes dominated the community specializing in straw-13C decomposition in soil containing both straw-C and rhizo-C; ii) the planted maize (i.e. rhizo-C input) changed community composition and metabolisms of straw-C utilizers, which shifted from K-strategists characterized by an enrichment of lignin-degrading genes to r-strategists which exhibited an enrichment of genes related to polysaccharide degradation. This metabolic shift of straw-C utilizer ultimately reduced straw-13C mineralization by 25.6% when maize was planted. This study identified the distinct utilizers of rhizo-C and straw-C in soils containing both C sources, and shed light on the shift of bacterial community and their metabolic activities responding to the changes of maize-derived C sources.
KW - Agro-ecosystem
KW - Bacterial community
KW - Carbon metabolism
KW - Continuous CO labeling
KW - Rhizo-C/straw utilizers
KW - SIP-Metagenome
UR - http://www.scopus.com/inward/record.url?scp=85202941450&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2024.109578
DO - 10.1016/j.soilbio.2024.109578
M3 - Article
AN - SCOPUS:85202941450
VL - 198
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
M1 - 109578
ER -