Details
| Original language | English |
|---|---|
| Article number | 1361 |
| Journal | CATALYSTS |
| Volume | 11 |
| Issue number | 11 |
| Publication status | Published - 12 Nov 2021 |
Abstract
Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono-and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes in these fungi. The basidiomycete Cerrena unicolor, a known sesquiterpene producer, was in silico screened for sesquiterpene cyclases via homology Basic Local Alignment Search Tool searches. Cyclase genes identified were cloned and heterologously expressed in Escherichia coli Bl21 using pCOLD I as the expression vector. Ten cyclases were successfully produced and purified, and their identity was confirmed using amino acid sequencing of tryptic peptides by nano-liquid chromatography-high resolution-electrospray ionization-tandem mass spectrome-try. Gas chromatography/mass spectrometry analysis was applied to characterize these cyclases according to the formation of sesquiterpene hydrocarbons and oxidized terpenoids. Bioinformatic characterization and phylogenetic determination allowed for the classification of these diverse fungal enzymes. A representative single and a multi-product STS, respectively, were further analyzed for their dependency from divalent metal cations as a cofactor for the catalytic activity.
Keywords
- Cerrena unicolor, Heterolo-gous expression, Metal ion dependency, Phylogenetic analysis, Sesquiterpenes, Terpene cyclases
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
- Physical and Theoretical Chemistry
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In: CATALYSTS, Vol. 11, No. 11, 1361, 12.11.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Sesquiterpene Cyclases from the Basidiomycete Cerrena unicolor
AU - Püth, Nils
AU - Ersoy, Franziska
AU - Krings, Ulrich
AU - Berger, Ralf G.
N1 - Funding Information: Funding: The APC was funded by the Open Access fund of the Gottfried Wilhelm Leibniz Universität Hannover.
PY - 2021/11/12
Y1 - 2021/11/12
N2 - Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono-and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes in these fungi. The basidiomycete Cerrena unicolor, a known sesquiterpene producer, was in silico screened for sesquiterpene cyclases via homology Basic Local Alignment Search Tool searches. Cyclase genes identified were cloned and heterologously expressed in Escherichia coli Bl21 using pCOLD I as the expression vector. Ten cyclases were successfully produced and purified, and their identity was confirmed using amino acid sequencing of tryptic peptides by nano-liquid chromatography-high resolution-electrospray ionization-tandem mass spectrome-try. Gas chromatography/mass spectrometry analysis was applied to characterize these cyclases according to the formation of sesquiterpene hydrocarbons and oxidized terpenoids. Bioinformatic characterization and phylogenetic determination allowed for the classification of these diverse fungal enzymes. A representative single and a multi-product STS, respectively, were further analyzed for their dependency from divalent metal cations as a cofactor for the catalytic activity.
AB - Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono-and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes in these fungi. The basidiomycete Cerrena unicolor, a known sesquiterpene producer, was in silico screened for sesquiterpene cyclases via homology Basic Local Alignment Search Tool searches. Cyclase genes identified were cloned and heterologously expressed in Escherichia coli Bl21 using pCOLD I as the expression vector. Ten cyclases were successfully produced and purified, and their identity was confirmed using amino acid sequencing of tryptic peptides by nano-liquid chromatography-high resolution-electrospray ionization-tandem mass spectrome-try. Gas chromatography/mass spectrometry analysis was applied to characterize these cyclases according to the formation of sesquiterpene hydrocarbons and oxidized terpenoids. Bioinformatic characterization and phylogenetic determination allowed for the classification of these diverse fungal enzymes. A representative single and a multi-product STS, respectively, were further analyzed for their dependency from divalent metal cations as a cofactor for the catalytic activity.
KW - Cerrena unicolor
KW - Heterolo-gous expression
KW - Metal ion dependency
KW - Phylogenetic analysis
KW - Sesquiterpenes
KW - Terpene cyclases
UR - http://www.scopus.com/inward/record.url?scp=85118893457&partnerID=8YFLogxK
U2 - 10.3390/catal11111361
DO - 10.3390/catal11111361
M3 - Article
VL - 11
JO - CATALYSTS
JF - CATALYSTS
SN - 2073-4344
IS - 11
M1 - 1361
ER -