Details
| Original language | English |
|---|---|
| Pages (from-to) | 437-444 |
| Number of pages | 8 |
| Journal | JAOCS, Journal of the American Oil Chemists' Society |
| Volume | 86 |
| Issue number | 5 |
| Publication status | Published - 31 Mar 2009 |
Abstract
Two extracellular esterases were produced in submerged cultures of the basidiomycete Pleurotus sapidus. A foam fractionation device was designed and employed for the isolation of the esterolytic enzymes. The recovery of enzyme activity in the liquefied foam strongly depended on the superficial gas velocity. High purification and enrichment factors (E a = 62.0, P = 15.5) were achieved using nitrogen at 1.87 cm min-1 within 100 min. Increasing the superficial gas velocity to 2.49 cm min-1 improved the recovery of total esterase activity in the foam to >95% at the expense of reduced enrichment and purification factors. Differences in their physicochemical characteristics resulted in differing foaming properties of the two esterases secreted by P. sapidus. By variation of the pH value of the culture medium and addition of Triton X-100, both esterases were successively and quantitatively transferred into the foam in a two-step fractionation process.
Keywords
- Basidiomycete, Downstream processing, Esterase, Foam fractionation, Pleurotus sapidus
ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemistry(all)
- Organic Chemistry
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In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 86, No. 5, 31.03.2009, p. 437-444.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Separation of extracellular esterases from pellet cultures of the basidiomycete pleurotus sapidus by foam fractionation
AU - Linke, Diana
AU - Nimtz, Manfred
AU - Berger, Ralf G.
AU - Zorn, Holger
N1 - Funding information: Acknowledgments Financial support by the AIF (project #121 ZN) through the Forschungskreis der Ernährungsindustrie e.V. (Bonn, Germany) is gratefully acknowledged. The project is part of the joint initiative ‘‘Biologisch aktive Naturstoffe-Chemische Diversität’’ at the Gottfried Wilhelm Leibniz University of Hannover.
PY - 2009/3/31
Y1 - 2009/3/31
N2 - Two extracellular esterases were produced in submerged cultures of the basidiomycete Pleurotus sapidus. A foam fractionation device was designed and employed for the isolation of the esterolytic enzymes. The recovery of enzyme activity in the liquefied foam strongly depended on the superficial gas velocity. High purification and enrichment factors (E a = 62.0, P = 15.5) were achieved using nitrogen at 1.87 cm min-1 within 100 min. Increasing the superficial gas velocity to 2.49 cm min-1 improved the recovery of total esterase activity in the foam to >95% at the expense of reduced enrichment and purification factors. Differences in their physicochemical characteristics resulted in differing foaming properties of the two esterases secreted by P. sapidus. By variation of the pH value of the culture medium and addition of Triton X-100, both esterases were successively and quantitatively transferred into the foam in a two-step fractionation process.
AB - Two extracellular esterases were produced in submerged cultures of the basidiomycete Pleurotus sapidus. A foam fractionation device was designed and employed for the isolation of the esterolytic enzymes. The recovery of enzyme activity in the liquefied foam strongly depended on the superficial gas velocity. High purification and enrichment factors (E a = 62.0, P = 15.5) were achieved using nitrogen at 1.87 cm min-1 within 100 min. Increasing the superficial gas velocity to 2.49 cm min-1 improved the recovery of total esterase activity in the foam to >95% at the expense of reduced enrichment and purification factors. Differences in their physicochemical characteristics resulted in differing foaming properties of the two esterases secreted by P. sapidus. By variation of the pH value of the culture medium and addition of Triton X-100, both esterases were successively and quantitatively transferred into the foam in a two-step fractionation process.
KW - Basidiomycete
KW - Downstream processing
KW - Esterase
KW - Foam fractionation
KW - Pleurotus sapidus
UR - http://www.scopus.com/inward/record.url?scp=67349192134&partnerID=8YFLogxK
U2 - 10.1007/s11746-009-1369-4
DO - 10.1007/s11746-009-1369-4
M3 - Article
AN - SCOPUS:67349192134
VL - 86
SP - 437
EP - 444
JO - JAOCS, Journal of the American Oil Chemists' Society
JF - JAOCS, Journal of the American Oil Chemists' Society
SN - 0003-021X
IS - 5
ER -