Details
| Original language | English |
|---|---|
| Article number | 000817 |
| Pages (from-to) | 929-952 |
| Number of pages | 24 |
| Journal | Microbiology |
| Volume | 165 |
| Issue number | 9 |
| Publication status | Published - 1 Sept 2019 |
| Externally published | Yes |
Abstract
Streptomyces venezuelae is a Gram-positive, filamentous actinomycete with a complex developmental life cycle. Genomic analysis revealed that S. venezuelae encodes a large number of two-component systems (TCSs): these consist of a membrane-bound sensor kinase (SK) and a cognate response regulator (RR). These proteins act together to detect and respond to diverse extracellular signals. Some of these systems have been shown to regulate antimicrobial biosynthesis in Streptomyces species, making them very attractive to researchers. The ability of S. venezuelae to sporulate in both liquid and solid cultures has made it an increasingly popular model organism in which to study these industrially and medically important bacteria. Bioinformatic analysis identified 58 TCS operons in S. venezuelae with an additional 27 orphan SK and 18 orphan RR genes. A broader approach identified 15 of the 58 encoded TCSs to be highly conserved in 93 Streptomyces species for which high-quality and complete genome sequences are available. This review attempts to unify the current work on TCS in the streptomycetes, with an emphasis on S. venezuelae.
Keywords
- Anti-Bacterial Agents/biosynthesis, Evolution, Molecular, Extracellular Signal-Regulated MAP Kinases/genetics, Genes, Regulator, Genomics, Regulatory Elements, Transcriptional/genetics, Response Elements/genetics, Signal Transduction/genetics, Streptomyces/genetics
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
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In: Microbiology, Vol. 165, No. 9, 000817, 01.09.2019, p. 929-952.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Sensing and responding to diverse extracellular signals
T2 - an updated analysis of the sensor kinases and response regulators of Streptomyces species
AU - McLean, Thomas C
AU - Lo, Rebecca
AU - Tschowri, Natalia
AU - Hoskisson, Paul A
AU - Al Bassam, Mahmoud M.
AU - Hutchings, Matthew I.
AU - Som, Nicolle F.
N1 - Funding information: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) via responsive mode grant BB/P005292/1 awarded to M. I. H. and Norwich Research Park BBSRC Postdoctoral Training Program Studentships BB/M011216/1 (T. C. M. and R. L.).
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Streptomyces venezuelae is a Gram-positive, filamentous actinomycete with a complex developmental life cycle. Genomic analysis revealed that S. venezuelae encodes a large number of two-component systems (TCSs): these consist of a membrane-bound sensor kinase (SK) and a cognate response regulator (RR). These proteins act together to detect and respond to diverse extracellular signals. Some of these systems have been shown to regulate antimicrobial biosynthesis in Streptomyces species, making them very attractive to researchers. The ability of S. venezuelae to sporulate in both liquid and solid cultures has made it an increasingly popular model organism in which to study these industrially and medically important bacteria. Bioinformatic analysis identified 58 TCS operons in S. venezuelae with an additional 27 orphan SK and 18 orphan RR genes. A broader approach identified 15 of the 58 encoded TCSs to be highly conserved in 93 Streptomyces species for which high-quality and complete genome sequences are available. This review attempts to unify the current work on TCS in the streptomycetes, with an emphasis on S. venezuelae.
AB - Streptomyces venezuelae is a Gram-positive, filamentous actinomycete with a complex developmental life cycle. Genomic analysis revealed that S. venezuelae encodes a large number of two-component systems (TCSs): these consist of a membrane-bound sensor kinase (SK) and a cognate response regulator (RR). These proteins act together to detect and respond to diverse extracellular signals. Some of these systems have been shown to regulate antimicrobial biosynthesis in Streptomyces species, making them very attractive to researchers. The ability of S. venezuelae to sporulate in both liquid and solid cultures has made it an increasingly popular model organism in which to study these industrially and medically important bacteria. Bioinformatic analysis identified 58 TCS operons in S. venezuelae with an additional 27 orphan SK and 18 orphan RR genes. A broader approach identified 15 of the 58 encoded TCSs to be highly conserved in 93 Streptomyces species for which high-quality and complete genome sequences are available. This review attempts to unify the current work on TCS in the streptomycetes, with an emphasis on S. venezuelae.
KW - Anti-Bacterial Agents/biosynthesis
KW - Evolution, Molecular
KW - Extracellular Signal-Regulated MAP Kinases/genetics
KW - Genes, Regulator
KW - Genomics
KW - Regulatory Elements, Transcriptional/genetics
KW - Response Elements/genetics
KW - Signal Transduction/genetics
KW - Streptomyces/genetics
UR - http://www.scopus.com/inward/record.url?scp=85071787092&partnerID=8YFLogxK
U2 - 10.1099/mic.0.000817
DO - 10.1099/mic.0.000817
M3 - Article
C2 - 31334697
VL - 165
SP - 929
EP - 952
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 9
M1 - 000817
ER -